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Volume 146 (1985)
- Issue 4 Pages 379-
- Issue 3 Pages 253-
- Issue 2 Pages 123-
- Issue 1 Pages 1-
Volume 146, Issue 2
Displaying 1-16 of 16 articles from this issue
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TOSHIO NISHIDE, NORIHIRO SASAKI, KOHJI SHIRAI, YASUSHI SAITO, SHO YOSH ...1985 Volume 146 Issue 2 Pages 123-130
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNISHIDE, T., SASAKI, N., SHIRAI, K., SAITO, Y. and YOSHIDA, S. Studies on Cholesterol Esterase in Cultured Rabbit Smooth Muscle Cells. Tohoku J. exp. Med., 1985, 146 (2), 123-130-Acid and neutral cholesterol esterases (CEases) in cultured rabbit smooth muscle cells (SMC) were examined. The optimal pH of acid CEase was 4.5 and its apparent Km was 83μM. The optimal pH of neutral CEase was 7.5 and its apparent Km was 38μM. CEases were most active with substrate vesicles prepared with phosphatidylcholine. Acid CEase hydrolyzed cholesteryl [1-14C]oleate labeled LDL (LDL-CE), but neutral CEase did not. Incubation with LDL increased the acid and neutral CEase activities of SMC, but incubation with acetylated-LDL did not increase their activities.View full abstractDownload PDF (562K) -
RYUICHI SANO, RYUZO ABE, SHIN-ICHI OIKAWA, YUTAKA FUJII, TAKAYOSHI TOY ...1985 Volume 146 Issue 2 Pages 131-136
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSANO, R., ABE, R., OIKAWA, S., FUJII, Y. and TOYOTA, T. ApoE-2/E-3 Ratio of Very Low Density Lipoprotein in Diabetes Mellitus. Tohoku J. exp. Med., 1985, 146(2), 131-136-Apolipoprotein E (apoE) of very low density lipoprotein (VLDL) in 22 diabetic patients was analysed by the method of one-dimensional isoelectrofocussing (IEF). We modified the IEF-method of Kashyap et al. by which apoE isoproteins were easily and accurately determined. The measurement of apoE-2/E-3 ratio was useful for determining apoE phenotypes. In 17 of 22 diabetics apoE phenotype was E3/3, while three patients showed E2/3 and two showed E3/4. ApoE-2/E-3 ratio was reduced from 0.91±0.07 to 0.73± 0.04 (p<0.05) by the one month-period treatment in 10 insulin required diabetics. The ratio was also reduced from 1.06±0.14 to 0.89±0.09 (p<0.2) in 10 non-insulin required diabetics. The reduction in apoE-2/E-3 ratio was caused by an increase in apoE-3 band and a decrease in apoE-2 band which might be attributed to the changes in the sialic acid-content of isoproteins on the one-dimensional IEF method. Therefore, apoE phenotypes in diabetic patients should be determined when their diabetic state is improved.View full abstractDownload PDF (626K) -
MASAO AZUMA1985 Volume 146 Issue 2 Pages 137-148
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAZUMA, M. Response Properties of Prefrontal Visual Neurons in the Awake Monkeys. Tohoku J. exp. Med., 1985, 146 (2), 137-148-Response properties of the visual neurons in the prearcuate and periprincipal areas of the prefrontal cortex were examined in awake monkeys. The findings obtained are as follows: (1) Most of the visual neurons responded with various excitatory fashions to a light spot presented in their visual receptive fields (RFs). The response was large when the stimulus was applied to the middle of the RF and became smaller when the stimulus position was moved from this part toward the RF border. (2) The response was affected by the size of the stimulus. The largest response was usually obtained by a small stimulus. The shape, orientation, brightness of the stimulus and the direction of its movement gave little influence on the response strength and pattern. (3) In about a half of the neurons the response was enhanced when the monkey made a saccadic eye-movement toward a visual stimulus in the RF. (4) Some of the above-mentioned response properties were quantitatively different from one another among neuron groups classified according to the eccentricity and size of their RFs. These results suggest that the properties of the neurons are suitable for detecting a visual stimulus occupying a limited extent in the space.View full abstractDownload PDF (1200K) -
SHUICHI ICHIKAWA, ZENPEI ONO, MASASHI ARAI, KAZUHIKO MURATA1985 Volume 146 Issue 2 Pages 149-152
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSICHIKAWA, S., ONO, Z., ARAI, M., and MURATA, K. Increase in Blood Pressure in Pheochromocytoma Induced by Domperidone and Metoclopramide. Tohoku J. exp. Med., 1985, 146 (2), 149-152-Administration of two dopamie antagonists, domperidone and metoclopramide, gave a marked rise in blood pressure with an increase in plasma catecholamines in two patients with pheochromocytoma. The result suggests that not only metoclopramide but also domperidone might induce hypertensive crisis in pheochromocytoma.View full abstractDownload PDF (279K) -
YOSHIMASA TASAKA, YUKIKO INOUE, KOJI MARUMO, YOSHIATSU MIZUNO, YUKIMAS ...1985 Volume 146 Issue 2 Pages 153-162
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTASAKA, Y., INOUE, Y., MARUMO, K., HIRATA, T. and AKAO, Y. Insulin, Glucagon and Somatostatin Content of the Non-Obese Diabetic (NOD) Mouse Pancreas and Plasma Virus Antibodies to Coxsackie B- and Reoviruses. Tohoku J. exp. Med., 1985, 146 (2), 153-162-The amounts of insulin, glucagon and somatostatin in the pancreas of NOD mice were determined and the results were compared with those of normal ICR-strain mice, and plasma antibodies to Coxsackie B-3 and reovirus types 1, 2 and 3 were measured. In the pancreas of NOD mice with fasting plasma glucose (FPG) less than 140mg/100ml, the insulin content of the male mice was similar to that of the normal controls, ranging to 3.55±0.31U/g wet weight of pancreas, but it was already significantly decreased to 0.85±0.52U/g in the female mice. In the NOD mice with FPG more than 201 mg/100ml, it was 0.002±0.001U/g. The glucagon content of the pancreas was 7.76±0.89μg/g in the normal controls and it was decreased slightly in the NOD mice, but the values among the NOD mice were not significantly different. Pancreas somatostatin showed a tendency to be higher in the NOD mice with FPG more than 201mg/100 ml. Histologically cell infiltration into the pancreatic islets was conspicious in the hyperglycemic NOD mice, but it was found even in the normoglycemic NOD. Plasma antibodies to Coxsackie B-3 Virus and reovirus types 1, 2 and 3 were not detected at any stage of FPG.View full abstractDownload PDF (3468K) -
KAORU SAGISAKA, TSUYOSHI YOKOI, TOSHIYUKI KUDO, MASAYASU NOGUCHI1985 Volume 146 Issue 2 Pages 163-166
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAGISAKA, K., YOKOI, T., KUDO, T. and NOGUCHI, M. Semiquantitation of ABH Antigens Specific for Red Blood Cells. Tohoku J. exp. Med., 1985, 146 (2), 163-166-H and A antigens expressed only in the RBC were quantitated using specific antisera, and the amount of the antigens was compared with that of total H and A antigens shared by the RBC and saliva. Total H antigen decreased in the order of O, A, B and AB blood groups. However, H antigen specific for the RBC distributed similarly among four blood groups. In addition, the amount of total A antigen in A group was more than that of AB group, but the difference in the amount of A antigen specific for the RBC was not significant between the two blood groups. B antigen specific for the RBC was not examined because of failure in preparing specific anti-B serum. In conclusion, the quantitative difference of the total H or A between A and AB groups might be due to the difference in the amount of antigen common to the RBC and saliva but not one specific for the RBC.View full abstractDownload PDF (628K) -
SHIN-ICHI OIKAWA, YOSHISUKE MARUHAMA, YOSHIO GOTO1985 Volume 146 Issue 2 Pages 167-174
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOIKAWA, S., MARUHAMA, Y. and GOTO, Y. Evidence for Direct Effect of Growth Hormone on Capillary Basement Membrane Thickness. Tohoku J. exp. Med., 1985, 146 (2), 167-174-In order to investigate the direct effect of growth hormone on the capillary basement membrane thickness (CBMT), we performed, in normal and diabetic rats, ultrastructural morphometric studies on the capillaries of the skin loci where the intracutaneous injections of physiologic saline and human growth hormone (HGH) in various concentrations had been repeated daily for 28 days. In the normal group, the injection (0.1ml daily) of HGH solutions in concentrations of 0.5μg/ml and 2μg/ml did not significantly alter the CBMT, and only at the concentration of 10μg/ml, HGH significantly (p<0.001) increased the CBMT. In the diabetic group, however, the injection of any of the HGH solutions significantly (p<0.001-0.05) increased the CBMT. In another diabetic rat series, HGH, human albumin, humam menopausal gonadotropin and human globulin (each in a concentration of 10μg/ml) were repeatedly injected into four separate skin portions for 28 days, and it was found that the net increase in CBMT was most striking in the HGH-treated portion. These data demonstrated the direct and specific effect of HGH on CBMTView full abstractDownload PDF (1039K) -
MASAKAZU OYA, REIKO SHIBATA, AKIRA KIDO, NORI KOMATSU1985 Volume 146 Issue 2 Pages 175-179
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOYA, M., SHIBATA, R., KIDO, A. and KOMATSU, N. A Further Study on the Polymorphism of Placental Alkaline Phosphatase in Japanese. Tohoku J. exp. Med., 1985, 146 (2), 175-179-Polymorphism of placental alkaline phosphatase (PL) was investigated in 120 Japanese placentae by means of starch gel electrophoresis and an improved staining method for the isoenzymes using naphthol AS-MX phosphate as substrate instead of β-naphthyl phosphate. The present method gave more satisfactory results for the detection of the PL bands. Besides five of the six common phenotypes three different uncommon variants were identified. The gene frequencies of PL types were estimated at PL1=0.729, PL2=0.054, PL3=0.200 and PLV=0.017, which were very similar to those reported in other districts of Japan.View full abstractDownload PDF (950K) -
RYOJI MINAMI, KOOJI WAGATSUMA, YUKITOSHI ISHIKAWA, MUTSUKO WATANABE, T ...1985 Volume 146 Issue 2 Pages 181-187
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMINAMI, R., WAGATSUMA, K., ISHIKAWA, Y., WATANABE, M., KUSANO, T., NAKAO, T. and TSUGAWA, S. Complementation Analysis ofβ-Galactosidase Deficiency by Means of Histochemical Method. Tohoku J. exp. Med., 1985, 146(2), 181-187-Cultured skin fibroblasts from 4 β-galactosidase-deficient patients with different clinical features were hybridized andβ-galactosidase activities were measured using 4-methylumbelliferyl (4MU)-derivate and by means of indigogenic method. Compared with the assays of β-galactosidase activity using 4MU-derivate, more clear-cut evidence was obtained in the indigogenic method for a judgement of complementation.View full abstractDownload PDF (2035K) -
MASAKAZU OYA, REIKO SHIBATA, AKIRA KIDO, NORI KOMATSU1985 Volume 146 Issue 2 Pages 189-192
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOYA, M., SHIBATA, R., KIDO, A. and KOMATSU, N. Polymorphism of Placental Phosphoglucomutase in Japanese. Tohoku J. exp. Med., 1985, 146 (2), 189-192-Polymorphism of PGM1 and PGM3 types was investigated in placental extracts from 127 unrelated Japanese parturients living in Yamanashi Prefecture. The gene frequencies were 0.787 for PGM11, 0.213 for PGM21; 0.760 for PGM13 0.240 for PGM23, respectively. There was no significant association between the PGM1 and PGM3 systems. The PGM23 frequency in the present sample population was higher than those in Kanto District, but lower than those in Kinki District.View full abstractDownload PDF (398K) -
KOICHIRO SAKAI, SADAO TAKASE, KATSUO KUMAGAI, FUMIO SHIMIZU1985 Volume 146 Issue 2 Pages 193-199
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAKAI, K., TAKASE, S., KUMAGAI, K. and SHIMIZU, F. Decreased Binding of Insulin to Erythrocytes in Myotonic Dystrophy. Tohoku J. exp. Med., 1985, 146 (2), 193-199-Recent several studies have shown that the insulin resistance exists in patients with myotonic dystrophy. Using in vitro radioreceptor assay with 125I-labeled insulin, we evaluated the binding of insulin to erythrocytes in patients with myotonic dystrophy. It was found that there was a decreased binding of insulin to erythrocytes in the patients with myotonic dystrophy. On quantitative analysis, this decrease in binding was found to be the result of a decrease in receptor affinity rather than receptor concentration. There was no evidence that the alteration in insulin receptor affinity was due to hyperinsulinemia. All of 7 patients with myotonic dystrophy also were shown to have an excessive response of endogenous insulin to a glucose challenge. No circulating antibody to insulin was found. These data suggest that a decrease in affinity of insulin receptors may, in part, cause insulin resistance and a compensatory elevation of the plasma insulin concentration in patients with myotonic dystrophy.View full abstractDownload PDF (630K) -
HIROKO KOSAKA, MAMORU ISEMURA, ZENSAKU YOSIZAWA, TERUO ONO1985 Volume 146 Issue 2 Pages 201-210
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOSAKA, H., ISEMURA, M., YOSIZAWA, Z., and ONO, T., Glycosidase Activities in the Rat Liver Microsomes. Tohoku J. exp. Med., 1985, 146 (2), 201-210-The glycosidase activities in a rat liver microsome fraction were investigated in comparison with those in a submitochondrial fraction. The activities of β-N-acetylglucosaminidase, β-galactosidase and β-glycosidase in the former were distinguished from those in the latter on the basis of solubilization pattern, pH optimum, thermal stability and elution pattern of concanavalin A-Sepharose chromatography. It is suggested that these activities are not due to the contamination of lysosomal enzymes but intrinsic to microsomes.View full abstractDownload PDF (988K) -
KIYOAKI OUCHI, KENJI OKABE, YOSHIHIRO ASANUMA, KENJI KOYAMA, TOSHIO SA ...1985 Volume 146 Issue 2 Pages 211-223
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOUCHI, K., OKABE, K., ASAMURA, Y., KOYAMA, K. and SATO, T. Alternations of Liver Pathophysiology in Experimental Hepatic Failure Treated by Two Forms of Plasma Purification Procedure Tohoku J. exp. Med., 1985, 146(2) 211-223 -Plasma cross-circulation which resembles plasma exchange and plasma perfusion over charcoal and resin adsorbents were carried out using rats with galactosamine (GalN)-induced hepatic failure to investigate the effects of those plasma purification procedures on damaged liver function. Twenty-four hr after the injection of GalN, the plasma treatment procedures were performed at a plasma flow rate of 0.1ml/min for 6hr. Forty-eight hr after the injection of GalN, ATP synthesis in liver mitochondrial function, and ATP and total adenine nucleotide contents in the liver, which were markedly reduced by the GalN administration, were increased by both plasma cross-circulation and plasma perfusion over adsorbents. These results suggest that both plasma purification procedures improve the deterioration of mitochondrial phosphorylation activity and produce an augmented high energy status in the liver. In the histological study of the liver, volume ratio of hepatic parenchyma in the GalN-treated liver to that in the normal liver was significantly higher in rats treated with plasma perfusion than those with sham-perfusion. An efficient removal of the hepatotoxic metabolites from the plasma, therefore, decelerates the progress of GalN induced-liver tissue necrosis, if rats with GalN-induced hepatic failure are treated in an early stage.View full abstractDownload PDF (1290K) -
MASAYUKI IKEDA, TATSUHIRO OHTSUKI1985 Volume 146 Issue 2 Pages 225-235
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIKEDA, M. and OHTSUKI, T. Exposure Concentration Versus Environmental Concentration: A Field Survey in Organic Solvent Workplaces. Tohoku J. exp. Med., 1985, 146 (2), 225-235-The relationship was investigated between the environmental concentrations in workplaces and the concentrations in the breath zones of the workers therein. The former was measured in 47 organic solvent workplaces of various types taking both the grid-sampling strategy (Measurement A) and a spot-air sampling to detect possible maximum exposure (Measurement B), while the latter was determined by the application of personal samplers to 328 workers. Although a low yet significant correlation was observed between the results of Measurement A (i.e., the combination of the geometric mean and the geometric standard deviation of the environmental concentration) and the exposure concentration, excess exposure (i.e., over occupational exposure limit exposure) would occur in some workplaces when the occupational exposure limit itself be used as the limit value for the setting of criterion for environmental concentration. The analyses of the experiences suggest no excess exposure would be expected if half the occupational exposure limit be employed as the limit value.View full abstractDownload PDF (1064K) -
TOSHIO OHARA, HIROSHI OGATA, JUN-ICHI FUJIYAMA, YUJI MURATA, JUN-ICHIR ...1985 Volume 146 Issue 2 Pages 237-249
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHARA, T., OGATA, H., FUJIYAMA, J., MURATA, Y., ABE, J., KAKUTA, K., KAMEYAMA, S. and YOSHIDA, Y. Effects of Prostaglandin E1 Infusion in the Pre-Operative Management of Critical Congenital Heart Disease. Tohoku J. exp. Med., 1985, 146 (2), 237-249-Prostaglandin E1(PGE1) was administered to 27 infants in whom pulmonary or systemic blood flow was entirely or significantly dependent upon the patency of the ductus arteriosus. In 12 patients with pulmonary atresia or severe pulmonary stenosis, PGE1 infusion was followed by an improvement in hypoxemia and acidemia (group I). In 2 patients with left ventricular outflow-tract obstruction, PGE1 infusion was followed by an improvement in arterial blood pressure, peripheral perfusion and urine output (group II). In 5 patients with d-transposition of the great arteries and intact ventricular septum who had persistent severe hypoxemia after creation of an interatrial communication, PGE1 infusion improved the arterial oxygenation with dilatation of the ductus arteriosus (group III). Seven patients (3 of group I, 2 of group II and 2 of group III) failed to respond to PGE1. There were no fatal side effects. It is concluded that PGE1 therapy is highly effective in stabilizing pre-operative conditions of infants with ductus-dependent congenital herat disease.View full abstractDownload PDF (1084K) -
MUNESHIGE TOBITA, KYUYA KOGURE, HIROMI SATO, HITOSHI OHTOMO, KOICHIRO ...1985 Volume 146 Issue 2 Pages 251-252
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTOBITA, M., KOGURE, K., SATO, H., OHTOMO, H., KAWASHIMA, K. and IDO, T. Evaluation of Sequential Glucose Metabolism in Cerebral Ischemia Using A Chrono-autoradiographic Method. Tohoku J. exp Med., 1985, 146 (2), 251-252 -A chrono-autoradiographic technique (sequential double label 2-deoxyglucose method) was perfomed to evaluate postischemic regional glucose metabolism. Using this method, two sequential autoradiograms, one immediately after the stroke and one 7-8hr after the stroke, were obtained from the same brain slice. Comparison of the two images strongly suggested that the ischemic core and the regions adjacent to the core died, while the periphery recovered from the metabolic injury, and that postischemic metabolic abnormality itself didn't necessarily determine the fate of the ischemic tissue.View full abstractDownload PDF (471K)
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