The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Glycosidase Activities in the Rat Liver Microsomes
HIROKO KOSAKAMAMORU ISEMURAZENSAKU YOSIZAWATERUO ONO
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1985 Volume 146 Issue 2 Pages 201-210

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Abstract
KOSAKA, H., ISEMURA, M., YOSIZAWA, Z., and ONO, T., Glycosidase Activities in the Rat Liver Microsomes. Tohoku J. exp. Med., 1985, 146 (2), 201-210-The glycosidase activities in a rat liver microsome fraction were investigated in comparison with those in a submitochondrial fraction. The activities of β-N-acetylglucosaminidase, β-galactosidase and β-glycosidase in the former were distinguished from those in the latter on the basis of solubilization pattern, pH optimum, thermal stability and elution pattern of concanavalin A-Sepharose chromatography. It is suggested that these activities are not due to the contamination of lysosomal enzymes but intrinsic to microsomes.
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