The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Non-T, Non-B Acute Lymphocytic Leukemias: Cellular Origin Based on Molecular Analyses of Immunoglobulin and T-Cell α- and β-Chain Receptor Gene Rearrangements
MIHIRO OKABESHOZO MATSUSHIMATAKASI FUKUHARAMASANORI TANAKAKEISUKE SAKURADAMITSUAKI KAKINUMAISAO MAEKAWATAMOTSU MIYAZAKI
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1987 Volume 152 Issue 2 Pages 197-207

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Abstract

OKABE, M., MATSUSHIMA, S., FUKUHARA, T., TANAKA, M., SAKURADA, K., KAKINUMA, M., MAEKAWA, I. and MIYAZAKI, T. Non-T, Non-B Acute Lymphocytic Leukemias: Cellular Origin Based on Molecular Analyses of Immunoglobulin and T-Cell α- and β-Chain Receptor Gene Rearrangements. Tohoku J. exp. Med., 1987, 152 (2), 197-207 - Fifteen non-T, non-B acute lymphocytic leukemia (ALL) cases were investigated for determining cellular origin based on molecular (immunoglobulin and T-cell α-receptor (TcRα) and T-cell β-receptor (TCRβ) genes) and immunophenotypical analyses. As defined by monoclonal antibodies, they were classified into 2 groups; 12 cases as common ALL antigen (CALLA)-positive ALL and 3 cases as CALLA-negative ALL. Southern blot analysis revealed that 11 CALLA-positive ALL cases contained rearranged JH gene and 2 of them contained rearranged Jx genes, similar to recent views that most CALLA-positive leukemic cells are neoplastic B-cell precursors. One CALLA-positive ALL case, whose leukemic cells were also Leu-1 positive, showed no rearrangement of JH and TcRβ genes. On the other hand, non-T, non-B CALLA-negative ALL, so called null ALL, consisted of heterogenous groups with regard to lymphocyte differentiation and lineage; one out of 3 null ALL cases may be truely undifferentiated as shown neither JH nor TcRβ gene rearrangement, but other 2 cases showed either JH or TcRβ gene rearrangement. Dual rearrangements of Ig and TcRβ genes occur frequently in 3 out of 15 non-T, non-B ALL cases, but all cases of bigenotype showed no doubly marked profile and retained a completely fidelous immunophenotypic pattern. We further investigated the possibility that analysis of TcRα gene may be useful for determining cellular origin of non-T, non-B ALL leukemic cells.

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