Dependence of the Number of Platelets Maintaining Aerobic Metabolism on the Size of Storage Containers

TETSUO SHIMIZU; KEIKO KOUKETSU

doi:10.1620/tjem.156.91

TETSUO SHIMIZU, KEIKO KOUKETSU

Author information

Keywords: platelet, in vitro storage, metabolism, glycolysis, oxygen permeability

JOURNAL FREE ACCESS

1988 Volume 156 Issue 1 Pages 91-98

DOI https://doi.org/10.1620/tjem.156.91

Details

Published: 1988 Received: February 23, 1988 Available on J-STAGE: August 31, 2006 Accepted: August 23, 1988 Advance online publication: - Revised: -
Correction information
Date of correction: August 31, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : SHIMIZU, T. and KOUKETSU, K. Dependence of the Number of Platelets Maintaining Aerobic Metabolism on the Size of Storage Containers. Tohoku J. exp. Med., 1988, 156 (1), 91-98-In order to elucidate the role of the size (surface area) of the storage container in maintaining the oxidative metabolism of platelets during in vitro storage at 22°C, human platelets were stored as platelet concentrates (PC) with plasma in conventional polyvinylchloride plastic containers of various sizes for 3 days at 22°C. Oxygen permeability of the container was 1.5nmole/min/atm/cm². Levels of the partial oxygen pressure (P_O₂) of PC depended on both container size and total number of platelets. The larger the containers, the higher was the P_O₂. P_O₂ linearly decreased to 16mmHg with increasing platelet number. In well-oxygenated PC, 93% of the total ATP production was through the oxidative phosphorylation. With further increases in platelet number in the container, P_O₂ maintained low levels. The increases in glucose consumption and concomitant lactate production occurred to compensate the oxygen debt. The pH fall in PC depended on the degree of glycolysis. The partial carbon dioxide pressure (P_CO₂) increased with increasing platelet number. However, the larger the container, the lower was the P_CO₂. When PC were stored in 6 different-sized containers, the number of platelets, at which P_O₂ was 16mmHg, was correlated with the size of the container. The present data indicate that the amounts of oxygen entering a container, which depended on container size, may determine the number of platelets maintaining oxidative metabolism.
Right : In order to elucidate the role of the size (surface area) of the storage container in maintaining the oxidative metabolism of platelets during in vitro storage at 22°C, human platelets were stored as platelet concentrates (PC) with plasma in conventional polyvinylchloride plastic containers of various sizes for 3 days at 22°C. Oxygen permeability of the container was 1.5 nmole/min/atm/cm². Levels of the partial oxygen pressure (P_O₂) of PC depended on both container size and total number of platelets. The larger the containers, the higher was the P_O₂. P_O₂ linearly decreased to 16 mmHg with increasing platelet number. In well-oxygenated PC, 93% of the total ATP production was through the oxidative phosphorylation. With further increases in platelet number in the container, P_O₂ maintained low levels. The increases in glucose consumption and concomitant lactate production occurred to compensate the oxygen debt. The pH fall in PC depended on the degree of glycolysis. The partial carbon dioxide pressure (P_CO₂) increased with increasing platelet number. However, the larger the container, the lower was the P_CO₂. When PC were stored in 6 different-sized containers, the number of platelets, at which P_O₂ was 16 mmHg, was correlated with the size of the container. The present data indicate that the amounts of oxygen entering a container, which depended on container size, may determine the number of platelets maintaining oxidative metabolism.

Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -

Abstract

In order to elucidate the role of the size (surface area) of the storage container in maintaining the oxidative metabolism of platelets during in vitro storage at 22°C, human platelets were stored as platelet concentrates (PC) with plasma in conventional polyvinylchloride plastic containers of various sizes for 3 days at 22°C. Oxygen permeability of the container was 1.5 nmole/min/atm/cm². Levels of the partial oxygen pressure (P_O₂) of PC depended on both container size and total number of platelets. The larger the containers, the higher was the P_O₂. P_O₂ linearly decreased to 16 mmHg with increasing platelet number. In well-oxygenated PC, 93% of the total ATP production was through the oxidative phosphorylation. With further increases in platelet number in the container, P_O₂ maintained low levels. The increases in glucose consumption and concomitant lactate production occurred to compensate the oxygen debt. The pH fall in PC depended on the degree of glycolysis. The partial carbon dioxide pressure (P_CO₂) increased with increasing platelet number. However, the larger the container, the lower was the P_CO₂. When PC were stored in 6 different-sized containers, the number of platelets, at which P_O₂ was 16 mmHg, was correlated with the size of the container. The present data indicate that the amounts of oxygen entering a container, which depended on container size, may determine the number of platelets maintaining oxidative metabolism.

Corresponding author

Correction information

Register with J-STAGE for free!