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Effect of Insulin on the Growth of a Human Hepatoma Cell Line PLC/PRF/5: A Possible Role of Insulin Receptor
YOSHIHARU MOTOO, KENICHI KOBAYASHI, NOBU HATTORI
Author information
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YOSHIHARU MOTOO
The First Department of Internal Medicine, School of Medicine, Kanazawa University
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KENICHI KOBAYASHI
The First Department of Internal Medicine, School of Medicine, Kanazawa University
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NOBU HATTORI
The First Department of Internal Medicine, School of Medicine, Kanazawa University
JOURNAL
FREE ACCESS
1988 Volume 156 Issue 4 Pages 351-357
Details
- Published: 1988 Received: May 06, 1988 Available on J-STAGE: August 31, 2006 Accepted: October 19, 1988 Advance online publication: - Revised: -
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Correction information
Date of correction: August 31, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : MOTOO, Y., KOBAYASHI, K. and HATTORI, N. Effect of Insulin on the Growth of a Human Hepatoma Cell Line PLC/PRF/5: A Possible Role of Insulin Receptor. Tohoku J. exp. Med., 1988, 156 (4), 351-357-Insulin produced increases in cell number, DNA synthesis and alpha-fetoprotein secretion of a human hepatoma cell line PLC/PRF/5. The insulin-treated cells showed a decrease in binding of 125I-insulin. The decreased binding was due to the decreased numbers as well as the low affinity constant of insulin receptor. These data suggest that insulin is a growth factor for the human hepatoma cells and that insulin receptor plays a role in the growth-promoting effect of insulin.
Right : Insulin produced increases in cell number, DNA synthesis and alpha-fetoprotein secretion of a human hepatoma cell line PLC/PRF/5. The insulin-treated cells showed a decrease in binding of 125I-insulin. The decreased binding was due to the decreased numbers as well as the low affinity constant of insulin receptor. These data suggest that insulin is a growth factor for the human hepatoma cells and that insulin receptor plays a role in the growth-promoting effect of insulin.
Date of correction: August 31, 2006 Reason for correction: - Correction: CITATION Details: Wrong : 1) Alexander, J.J., Bey, E.M., Geddes, E.W. & Lecatsas, G. (1976) Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. med. J., 50, 2124-2128.
2) Gey, G.O. & Thalhimer, W. (1924) Observations on the effects of insulin introduced into the medium of tissue culture. J. Amer. med. Ass., 50, 1609-1609.
3) Hofmann, C., Marsh, J.W., Miller, B. & Steiner, D.F. (1980) Cultured hepatoma cells as a model system for studying insulin processing and biologic responsiveness. Diabetes, 29, 865-874.
4) Hollenberg, M.D. & Cuatrecasas, P. (1975) Insulin and epidermal growth factor. Human fibroblasts receptors related to deoxyribonucleic acid synthesis and amino acid synthesis and amino acid uptake. J. biol. Chem., 250, 3845-3853.
5) Iwamoto, Y., Wong, K.Y. & Goldfine, I.D. (1981) Insulin action in cultured HTC and H35 rat hepatoma cells: Receptor binding and hormone sensitivity. Endocrinology, 108, 44-51.
6) Jiménez de Asúa, L., Surian, E.S., Flawia, M.M. & Torres, H.N. (1973) Effect of insulin on the growth pattern and adenylate cyclase activity of BIIK fibroblasts. Proc. nat Acad. Sci. USA, 70, 1388-1392.
7) Kasuga, M., Hedo, J.A., Yamada, KM. & Kahn, C.R. (1982a). The, structure of insulin receptor and its subunits. J. biol. Chem., 257, 10392-10399.
8) Kasuga, M., Karlsson, F.A. & Kahn, C.R. (1982b) Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor. Science, 215, 185-187.
9) Kasuga, M., Yamaguchi, Y.F., Blithe, D.L. & Kahn, C.R. (1983) Tyrosine specific protein kinase activity is associated with the purified insulin receptor.. Proc. nat. Acad. Sci. USA, 80, 2137-2141.
10) King, G.L. & Kahn, C.R. (1981) Non-parallel evolution of metabolic and growth-promoting functions of insulin. Nature (Lond.), 292, 644-646.
11) Kolata, G. (1983) Is tyrosine the key to growth control? Science, 219, 377-378.
12) Koontz, J.W. & Iwahashi, M. (1981) Insulin as a potent growth factor in a rat hepatoma cell line. Science, 211, 947-949.
13) Leffert, H.L. (1974) Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VII. Hormonal control of DNA synthesis and its possible significance to the problem of liver regeneration. J. Cell Biol., 62, 792-801.
14) Moses, A.C., Freinkel, A.J., Knowles, B.B. & Aden, D.P. (1983) Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J. clin. Endocr., 56, 1003-1008.
15) Motoo, Y., Tanaka, N., Kitagawa, H., Kobayashi, K. & Hattori, N. (1988) Growth factor receptors in human hepatoma cell lines. Acta hepatol. jap., 29, 1423-1424. (Japanese).
16) Pezzino, V., Vigneri, R., Siperstein, M.D. & Goldfine, ID. (1979) Insulin and glucagon receptors in Morris hepatomas of varying growth rates. Cancer Res., 39, 1443-1446.
17) Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. N.Y. Acad. Sci., 51, 660-672.
18) Todaro, G.J., Fryling, C. & DeLarco, J.E. (1980) Transforming growth factors produced by certain human tumor cells: Polypeptides that interact with epidermal growth factor receptors. Proc. nat. Acad. Sci. USA, 77, 5258-5262.
Right : 1) Alexander, J. J., Bey, E. M., Geddes, E. W. & Lecatsas, G. (1976) Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. med. J., 50, 2124-2128.
2) Gey, G. O. & Thalhimer, W. (1924) Observations on the effects of insulin introduced into the medium of tissue culture. J. Amer. med. Ass., 50, 1609-1609.
3) Hofmann, C., Marsh, J. W., Miller, B. & Steiner, D. F. (1980) Cultured hepatoma cells as a model system for studying insulin processing and biologic responsiveness. Diabetes, 29, 865-874.
4) Hollenberg, M. D. & Cuatrecasas, P. (1975) Insulin and epidermal growth factor. Human fibroblasts receptors related to deoxyribonucleic acid synthesis and amino acid synthesis and amino acid uptake. J. biol. Chem., 250, 3845-3853.
5) Iwamoto, Y., Wong, K. Y. & Goldfine, I. D. (1981) Insulin action in cultured HTC and H35 rat hepatoma cells: Receptor binding and hormone sensitivity. Endocrinology, 108, 44-51.
6) Jiménez de Asúa, L., Surian, E. S., Flawia, M. M. & Torres, H. N. (1973) Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts. Proc. nat Acad. Sci. USA, 70, 1388-1392.
7) Kasuga, M., Hedo, J. A., Yamada, K. M. & Kahn, C. R. (1982a). The structure of insulin receptor and its subunits. J. biol. Chem., 257, 10392-10399.
8) Kasuga, M., Karlsson, F. A. & Kahn, C. R. (1982b) Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor. Science, 215, 185-187.
9) Kasuga, M., Yamaguchi, Y. F., Blithe, D. L. & Kahn, C. R. (1983) Tyrosine specific protein kinase activity is associated with the purified insulin receptor. Proc. nat. Acad. Sci. USA, 80, 2137-2141.
10) King, G. L. & Kahn, C. R. (1981) Non-parallel evolution of metabolic and growthpromoting functions of insulin. Nature (Lond.), 292, 644-646.
11) Kolata, G. (1983) Is tyrosine the key to growth control? Science, 219, 377-378.
12) Koontz, J. W. & Iwahashi, M. (1981) Insulin as a potent growth factor in a rat hepatoma cell line. Science, 211, 947-949.
13) Leffert, H. L. (1974) Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VII. Hormonal control of DNA synthesis and its possible significance to the problem of liver regeneration. J. Cell Biol., 62, 792-801.
14) Moses, A. C., Freinkel, A. J., Knowles, B. B. & Aden, D. P. (1983) Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J. clin. Endocr., 56, 1003-1008.
15) Motoo, Y., Tanaka, N., Kitagawa, H., Kobayashi, K. & Hattori, N. (1988) Growth factor receptors in human hepatoma cell lines. Acta hepatol. jap., 29, 1423-1424. (Japanese).
16) Pezzino, V., Vigneri, R., Siperstein, M. D. & Goldfine, I. D. (1979) Insulin and glucagon receptors in Morris hepatomas of varying growth rates. Cancer Res., 39, 1443-1446.
17) Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci., 51, 660-672.
18) Todaro, G. J., Fryling, C. & DeLarco, J. E. (1980) Transforming growth factors produced by certain human tumor cells: Polypeptides that interact with epidermal growth factor receptors. Proc. nat. Acad. Sci. USA, 77, 5258-5262.
Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -
Date of correction: August 31, 2006 Reason for correction: - Correction: CITATION Details: Wrong : 1) Alexander, J. J., Bey, E. M., Geddes, E. W. & Lecatsas, G. (1976) Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. med. J., 50, 2124-2128.
2) Gey, G. O. & Thalhimer, W. (1924) Observations on the effects of insulin introduced into the medium of tissue culture. J. Amer. med. Ass., 50, 1609-1609.
3) Hofmann, C., Marsh, J. W., Miller, B. & Steiner, D. F. (1980) Cultured hepatoma cells as a model system for studying insulin processing and biologic responsiveness. Diabetes, 29, 865-874.
4) Hollenberg, M. D. & Cuatrecasas, P. (1975) Insulin and epidermal growth factor. Human fibroblasts receptors related to deoxyribonucleic acid synthesis and amino acid synthesis and amino acid uptake. J. biol. Chem., 250, 3845-3853.
5) Iwamoto, Y., Wong, K. Y. & Goldfine, I. D. (1981) Insulin action in cultured HTC and H35 rat hepatoma cells: Receptor binding and hormone sensitivity. Endocrinology, 108, 44-51.
6) Jiménez de Asúa, L., Surian, E. S., Flawia, M. M. & Torres, H. N. (1973) Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts. Proc. nat Acad. Sci. USA, 70, 1388-1392.
7) Kasuga, M., Hedo, J. A., Yamada, K. M. & Kahn, C. R. (1982a). The structure of insulin receptor and its subunits. J. biol. Chem., 257, 10392-10399.
8) Kasuga, M., Karlsson, F. A. & Kahn, C. R. (1982b) Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor. Science, 215, 185-187.
9) Kasuga, M., Yamaguchi, Y. F., Blithe, D. L. & Kahn, C. R. (1983) Tyrosine specific protein kinase activity is associated with the purified insulin receptor. Proc. nat. Acad. Sci. USA, 80, 2137-2141.
10) King, G. L. & Kahn, C. R. (1981) Non-parallel evolution of metabolic and growthpromoting functions of insulin. Nature (Lond.), 292, 644-646.
11) Kolata, G. (1983) Is tyrosine the key to growth control? Science, 219, 377-378.
12) Koontz, J. W. & Iwahashi, M. (1981) Insulin as a potent growth factor in a rat hepatoma cell line. Science, 211, 947-949.
13) Leffert, H. L. (1974) Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VII. Hormonal control of DNA synthesis and its possible significance to the problem of liver regeneration. J. Cell Biol., 62, 792-801.
14) Moses, A. C., Freinkel, A. J., Knowles, B. B. & Aden, D. P. (1983) Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J. clin. Endocr., 56, 1003-1008.
15) Motoo, Y., Tanaka, N., Kitagawa, H., Kobayashi, K. & Hattori, N. (1988) Growth factor receptors in human hepatoma cell lines. Acta hepatol. jap., 29, 1423-1424. (Japanese).
16) Pezzino, V., Vigneri, R., Siperstein, M. D. & Goldfine, I. D. (1979) Insulin and glucagon receptors in Morris hepatomas of varying growth rates. Cancer Res., 39, 1443-1446.
17) Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci., 51, 660-672.
18) Todaro, G. J., Fryling, C. & DeLarco, J. E. (1980) Transforming growth factors produced by certain human tumor cells: Polypeptides that interact with epidermal growth factor receptors. Proc. nat. Acad. Sci. USA, 77, 5258-5262.
Right : 1) Alexander, J. J., Bey, E. M., Geddes, E. W. & Lecatsas, G. (1976) Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Aft. med. J., 50, 2124-2128.
2) Gey, G. O. & Thalhimer, W. (1924) Observations on the effects of insulin introduced into the medium of tissue culture. J. Amer. med. Ass., 50, 1609-1609.
3) Hofmann, C., Marsh, J. W., Miller, B. & Steiner, D. F. (1980) Cultured hepatoma cells as a model system for studying insulin processing and biologic responsiveness. Diabetes, 29, 865-874.
4) Hollenberg, M. D. & Cuatrecasas, P. (1975) Insulin and epidermal growth factor. Human fibroblasts receptors related to deoxyribonucleic acid synthesis and amino acid synthesis and amino acid uptake. J. biol. Chem., 250, 3845-3853.
5) Iwamoto, Y., Wong, K. Y. & Goldfine, I. D. (1981) Insulin action in cultured HTC and H35 rat hepatoma cells: Receptor binding and hormone sensitivity. Endocrinology, 108, 44-51.
6) Jiménez de Asúa, L., Surian, E. S., Flawia, M. M. & Torres, H. N. (1973) Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts. Proc. nat Acad. Sci. USA, 70, 1388-1392.
7) Kasuga, M., Hedo, J. A., Yamada, K. M. & Kahn, C. R. (1982a). The structure of insulin receptor and its subunits. J. biol. Chem., 257, 10392-10399.
8) Kasuga, M., Karlsson, F. A. & Kahn, C. R. (1982b) Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor. Science, 215, 185-187.
9) Kasuga, M., Yamaguchi, Y. F., Blithe, D. L. & Kahn, C. R. (1983) Tyrosine specific protein kinase activity is associated with the purified insulin receptor. Proc. nat. Acad. Sci. USA, 80, 2137-2141.
10) King, G. L. & Kahn, C. R. (1981) Non-parallel evolution of metabolic and growthpromoting functions of insulin. Nature (Lond.), 292, 644-646.
11) Kolata, G. (1983) Is tyrosine the key to growth control? Science, 219, 377-378.
12) Koontz, J. W. & Iwahashi, M. (1981) Insulin as a potent growth factor in a rat hepatoma cell line. Science, 211, 947-949.
13) Leffert, H. L. (1974) Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VII. Hormonal control of DNA synthesis and its possible significance to the problem of liver regeneration. J. Cell Biol., 62, 792-801.
14) Moses, A. C., Freinkel, A. J., Knowles, B. B. & Aden, D. P. (1983) Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J. clin. Endocr., 56, 1003-1008.
15) Motoo, Y., Tanaka, N., Kitagawa, H., Kobayashi, K. & Hattori, N. (1988) Growth factor receptors in human hepatoma cell lines. Acta hepatol. jap., 29, 1423-1424. (Japanese).
16) Pezzino, V., Vigneri, R., Siperstein, M. D. & Goldfine, I. D. (1979) Insulin and glucagon receptors in Morris hepatomas of varying growth rates. Cancer Res., 39, 1443-1446.
17) Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci., 51, 660-672.
18) Todaro, G. J., Fryling, C. & DeLarco, J. E. (1980) Transforming growth factors produced by certain human tumor cells: Polypeptides that interact with epidermal growth factor receptors. Proc. nat. Acad. Sci. USA, 77, 5258-5262.
Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -
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