The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
Monoclonal Antibody Defining Tax1 Protein of Human T-Cell Leukemia Virus Type-I
Author information

Volume 157 (1989) Issue 1 Pages 1-11

Download PDF (7390K) Contact us

LEE, B., TANAKA, Y. and TOZAWA, H. Monoclonal Antibody Defining Tax1 Protein of Human T-Cell Leukemia Virus Type-I. Tohoku J. Exp. Med., 1989, 157 (1), 1-11 - Weprepared a monoclonal antibody (MAb), Lt-4, which recognizes a tax1 protein expressed in HTLV-I-infected cells. Indirect immunofluorescence staining showed that the antigen recognized by Lt-4 MAb located mainly in the nuclei of HTLV-I-infected T cell lines such as HUT102, TCL-As2, MT-1 and MT-2, and also weakly in the cytoplasm of MT-2 cell line. Lt-4 MAb did not react with two HTLV-I-uninfected T cell lines tested and fresh and PHA-activated peripheral blood lymphocytes (PBL) of several normal donors. Furthermore, Lt-4 MAb stained the nuclei of HeLa cells infected with a recombinant vaccinia virus encoding the tax1 but not with a wild type vaccinia virus. In Western blot (WB) and radioimmunoprecipitation analyses, Lt-4 MAb detected a 40kDa molecule (p40) in HUT102 and TCL-As2 cells, and p40 and p68 in MT-2 cells. These results show that Lt-4 MAb is highly specific for the tax1 protein.

Information related to the author
© Tohoku University Medical Press
Next article

Recently visited articles
  • About this Journal
    The Tohoku Journal of Experimental Medicine (TJEM) was founded in 1920 by professors of Tohoku Imperial University, Medical School. The TJEM has been published continuously, except for the year of 1946 just after the World War II. The TJEM is open to original articles in all branches of medical sciences. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology.

  • Submitted manuscripts will be screened for plagiarism with Similarity Check (
Journal news & Announcements
  • Subscriptions
    Inland subscriptions should be sent to Tohoku University Medical Press, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JAPAN.

    Subscriptions from abroad should be addressed to MARUZEN CO., LTD., EXPORT DEPARTMENT, Postal address: P.O.Box 75, Shinagawa, Tokyo 140-8799, JAPAN.