LEE, B., TANAKA, Y. and TOZAWA, H. Monoclonal Antibody Defining Tax1Protein of Human T-Cell Leukemia Virus Type-I. Tohoku J. Exp. Med., 1989, 157 (1), 1-11 - Weprepared a monoclonal antibody (MAb), Lt-4, which recognizes a tax1 protein expressed in HTLV-I-infected cells. Indirect immunofluorescence staining showed that the antigen recognized by Lt-4 MAb located mainly in the nuclei of HTLV-I-infected T cell lines such as HUT102, TCL-As2, MT-1 and MT-2, and also weakly in the cytoplasm of MT-2 cell line. Lt-4 MAb did not react with two HTLV-I-uninfected T cell lines tested and fresh and PHA-activated peripheral blood lymphocytes (PBL) of several normal donors. Furthermore, Lt-4 MAb stained the nuclei of HeLa cells infected with a recombinant vaccinia virus encoding the tax1 but not with a wild type vaccinia virus. In Western blot (WB) and radioimmunoprecipitation analyses, Lt-4 MAb detected a 40kDa molecule (p40) in HUT102 and TCL-As2 cells, and p40 and p68 in MT-2 cells. These results show that Lt-4 MAb is highly specific for the tax1 protein.
FUJITA, M. and NAKAMURA, R. Characteristics of the Fastest Isometric KneeExtension in Patients with Spinocerebellar Degenerations. Tohoku J. Exp. Med., 1989, 157 (1), 13-17 - The force output of the knee extensor muscles and its time course were measured in nine patients with spinocerebellar degenerations and age-matched 10 normal subjects during the fastest and strongest isometric contraction. The time from the rise of tension to its maximum, FTmax, was definitely long in the SCD compared to the normal group, whereas the peak tension was not different between the two groups. FTmax of the SCD group was not related to the rate of tension development and the maximum tension nor to the degree of ataxia, suggesting that the prolongation of FTmax was a characteristic feature of cerebellar lesions.
KOIKE, K., ONO, S., SAKUMA, T., TANITA, T. and NAKADA, T. Collapse andReexpansion of Lungs Increase Microvascular Permeability in Sheep. Tohoku J. Exp. Med., 1989, 157 (1), 19-29 - The pathogenesis of reexpansion pulmonary edema has not been well studied. We tested the hypothesis that both long term collapse and subsequent reexpansion of the lungs cause reexpansion pulmonary edema by increasing pulmonary microvascular permeability. We investigated lymph dynamics in 15 experiments on collapsed lung and 10 experiments after lung reexpansion in 14 unanesthetized sheep with chronic lymph fistulas. We found that 24-hr left lung collapse increased lymph flow through the caudal mediastinal lymph node from the baseline of 1.71±0.97 (mean±S.D.)g/15min to 2.01±0.99g/15min, although 2-hr collapse did not affect lymph flow. The L/P ratio did not fall below baseline in either experiment. Pulmonary arterial pressure increased by only about 6cmH2O both in 2-hr and 24-hr collapse. Reexpansion after 24-hr lung collapse also increased lymph flow from the baseline of 1.64±0.52g/15min to 3.20±0.79g/15min during the first 2hr after reexpansion. The lymph-to-plasma protein concentration ratio did not fall below the baseline. Reexpansion after 2-hr collapse did not affect these variables. We conclude that both long term lung collapse and subsequent reexpansion lead to reexpansion pulmonary edema by increasing pulmonary microvascular permeability.
TAKEDA, K., SUZUKI, T., SUNAMURA, M., MATSUBARA, S., KOBARI, M. and MATSUNO, S. Effect of Blood Exchange Transfusion as an Initial Treatment ofAcute Hemorrhagic Pancreatitis. Tohoku J. Exp. Med., 1989, 157 (1), 31-37 -The effect of blood exchange transfusion on experimental acute pancreatitis was investigated. Acute hemorrhagic pancreatitis was induced in rats by the injection of sodium taurocholate into the pancreatic duct. One hour after the induction of pancreatitis, blood exchange transfusion was carried out in these rats. The survival rates by 24hr significantly increased in the exchange transfusion group as compared to those in the non-treated control group. Blood exchange transfusion, though had not significant effect on the macroscopic findings at autopsy, reduced pulmonary edema. These results suggested that blood purification by exchange transfusion may be beneficial for patients with acute pancreatitis, especially at an early stage.
ITO, M., HSU, C., SHIKUWA, S., KAWASE, Y., SEKINE, I. and FUJII, H. Multiple Myeloma in Alcoholic Liver Cirrhosis. Tohoku J. Exp. Med., 1989, 157 (1), 39-44 - A case of multiple myeloma (Bence Jones, lambda) associated with alcoholic liver cirrhosis is reported. A 56-year-old Japanese male died of hepatic failure and hypercalcemia. Autopsy revealed alcoholic liver cirrhosis and plasma cell myeloma. Immunoelectrophoretic analysis of his reserved serum disclosed the presence of M component of lambda Bence Jones protein. IgA and lambda light chain were demonstrated in the cytoplasm of the myeloma cells. Complications such as generalized amyloidosis, metastatic calcification, myeloma kidney and hemorrhagic pancreatitis were noted. The coexistence of multiple myeloma and liver cirrhosis has rarely been reported. On the basis of a review of the reported cases, a possible association between both diseases was discussed.
WATANABE, S.Y., HANDA, Y. and SHIMIZU, Y. The Effects of ElevatedPotassium on Sympathetic Ganglion Cells in Culture. Tohoku J. Exp. Med., 1989, 157 (1), 45-54 - Effects of a high potassium (40mM) medium on the survival and differentiation of sympathetic ganglion cells from chick embryos were studied in dissociated cell culture. In the high potassium medium, survival of the sympathetic ganglion neurons was improved and catecholamine fluorescence of the nerve fibers increased with several days in culture, while acetylcholinesterase activity was slightly positive. In contrast, in the control medium, catecholamine fluorescence was only faintly observed, while acetylcholinesterase became strongly positive. Catecholamine fluorescence was intensified by increasing the potassium concentration in a medium, while it was diminished by reversing the potassium level back to the normal one. The effect of the high potassium medium on catecholamine fluorescence was reduced by Ca++ influx inhibitors, diltiazem or Mg++. It is suggested that the high potassium medium increased the survival rate and prevented the sympathetic neurons from becoming cholinergic and allowed them to develop their adrenergic properties presumably through an increased level of the intracellular Ca++ due to Ca++ entry.
FUNAKOSHI, A., NAKANO, I. and MIYAZAKI, K. Vasoactive Intestinal PolypeptideStimulates Cholecystokinin Secretion in Perfused Rat Duodenum. Tohoku J. Exp. Med., 1989, 157 (1), 55-59 - We examined the effect of vasoactive intestinal polypeptide (VIP) on cholecystokinin (CCK) secretion from the isolated perfused rat duodenum. VIP stimulated CCK secretion mono-phasically in a concentration-dependent manner in concentrations ranging from 10-9 to 10-7M, and 10-7M of VIP led to an increment of 82±25.8fmole/3min. The stimulatory effect of VIP on CCK was not inhibited by 10-5M atropine. These results suggest that VIP may directly stimulate CCK secretion from the duodenum and work as a non-cholinergic, peptidergic neurotransmitter.
NAKAGAWA, K., MOMONO, S., SASAKI, Y., FURUSAWA, A. and UJIIE, K. Endoscopic Examination for Pancreatic Fistula. Tohoku J. Exp. Med., 1989, 157 (1), 61-67 - We have conducted endoscopic examination in 5 cases of postoperative pancreatic fistula, and discuss its clinical significance. The examination provided not only an observation of the fistula but also opportunity to irrigate the fistula for removal of foreign substances. The biopsy permitted histological examination for necrotic substances and the fistulous wall. Endoscopy made it easy to introduce a drain into the affected site and to judge when to remove the drain. Closure of the fistula was also attainable using fibrin glue. Consequently, the fistulas were closed within 1 month from the examination in all cases but one, in which death resulted due to carcinoma. The examination is an easy and safe technique.
ITAGAKI, Y., YOSHIDA, K., SAKURADA, T., KISO, Y., ARAI, T., KAISE, N., KAISE, K., HANEW, K., YAMAMOTO, M., SAITO, S. and YOSHINAGA, K. A Case of Refetoff Syndrome: Selective Venous Sampling for TSH is Useful in Differentiating Thyroid Hormone Resistance from TSH Secreting Tumor. Tohoku J. Exp. Med., 1989, 157 (1), 69-78 - A 22-year old man with a goiter and clinical manifestations of mild thyrotoxicosis (finger tremor, palpitation, tachycardia) was diagnosed as a syndrome of inappropriate secretion of TSH. Serum concentrations of T4, free T4, T3 and TSH were 24.1μg/100ml, 4.07ng/100ml, 261ng/100ml and 1.72 μU/ml, respectively. Thyroidal 131I uptake at 24hr was 80%. The BMR was within the normal range. He had a normal TSH response to TRH (500μg) with a peak level of 23.8μU/ml. The basal level of alpha-subunit of TSH was not elevated (0.35ng/ml). Oral 1-T3 administration (75 and 150μg daily) raised serum T3 concentration, reduced basal TSH and blunted TSH response to TRH. The diurnal variation of TSH was maintained. There was no evidence of abnormalities in the secretion of other pituitary hormones. These findings were compatible with thyroid hormone resistance. However, the presence of a microadenoma in the pituitary gland was suspected with CT scan. Bilateral and simultaneous venous sampling for TSH from inferior petrosal sinus showed no gradient in TSH concentration indicating that a TSH secreting pituitary tumor was unlikely. These data suggest that inappropriate TSH secretion in the present patient is resulted from resistance to thyroid hormone. In the present study selective venous sampling is useful to differentiate the thyroid hormone resistance from a TSH secreting tumor.
HIDA, W., TAGUCHI, O., IWASE, N., SATO, M., CHONAN, T., KIKUCHI, Y., OGATA, M., SHIRATO, K. and TAKISHIMA, T. Change in P Wave Height duringProgressive Exercise in Patients with Chronic Obstructive Pulmonary Disease. Tohoku J. Exp. Med., 1989, 157 (1) 79-80 - We examined changes in P wave height in lead II of electrocardiogram during progressive exercise in patients with chronic obstructive pulmonary disease (COPD), and obtained the slope (ΔP/ΔVO2, %/ml/min) of the regression line calculated from the relationship between percent change of P wave height and oxygen consumption (VO2). Four COPD patients, who had a decrease over 5mmHg in arterial oxygen tension (PaO2) at maximal exercise (group A), had significantly greater slope (0.45±0.14%/ml/min, mean±S.E.) than in five COPD patients (group B, 0.14±0.05), who did not have a decrease over 5mmHg in PaO2 at maximal exercise. The increase in P wave height during exercise was inhibited by oxygen inhalation at the given VO2 in group A. These findings suggest that increase in P wave height during exercise in COPD patients may be correlated with hypoxemia during exercise.
OHE, M., OGATA, M., SHIRATO, K. and TAKISHIMA, T. Effects of Verapamil and BAY K 8644 on the Hypoxic Contraction of the Isolated Human Pulmonary Artery. Tohoku J. Exp. Med., 1989, 157 (1), 81-82 - To examine the effect of hypoxia on the human pulmonary artery (HPA), HPA segments (2mm, o.d.) were suspended and changes in isometric force with hypoxia (PO2=44±3mmHg, mean±S.E.) were measured. HPA contracted with hypoxia and the tension developed with 15min of hypoxia was 127±36 (mean±S.E.) mg. Verapamil at 10-6M inhibited and 10-6M BAY K 8644 enhanced the hypoxic response by 47± 10% (p<0.01) and 396±106% (p<0.05), respectively, compared with preceding values. These results show that activation of voltage-dependent Ca2+ channels also plays an important role in the mechanism of hypoxic pulmonary vasoconstriction in man