Abstract
KIKUCHI, H., SEKIYA, T., NISHIMURA, S. and WATANABE, M. Repetitive Sequences in the Rat Genome that Have Cleavage Sites for the Restriction Endonuclease Taq I. Tohoku J. Exp. Med., 1990, 161 (2), 91-99-A cleavage of rat DNA with restriction endonuclease Tag I produced at least 8 discrete bands. One of the bands, 0.29 kilobase pairs (kb) long, was isolated and cloned in plasmid. The hybridization of this fragment to genomic DNA digested with Taq I showed 1.6kb, 1.2kb and 0.29kb band in a smear hybridization signal. A repetition of the DNA sequence of this fragment was about 12, 500 copies haploid genome of both Buffalo and Sprague-Dawley rats by the Cot-hybridization analysis. Four independent clones of 0.29kb Taq I fragment share high homology (97%).
