Abstract
KIKUCHI, Y., KOIZUMI, M., SHIMOSEGAWA, T., KASHIMURA, J., SUZUKI, S. and TOYOTA, T. Islet Amyloid Polypeptide has No Effect on Amylase Release from Rat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP. Tohoku J. Exp. Med., 1991, 165 (1), 41-48-Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11M), secretin (0.5×10-6M) or carbachol (10-6M), or by a maximal dose of vasoactive intestinal peptide (10-8M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.
