KIKUCHI, K., KOWADA, M., SASANUMA, J., OGAYAMA, H. and WATANABE, K. Local Image Filing System in Neurosurgery: Validity of Selective Storage ofRadiographic Images. Tohoku J. Exp. Med., 1991, 165 (1), 1-11-In an effort to achieve efficient image management in the practice of neurosurgery, a clinical data base system was designed and developed to process, store and retrieve neuroradiologic image information on hospitalized patients with the use of a local image filing system (EFPACS-500, Fuji Electric Co., Ltd). Storage and retrieval of radiographic images were studied to quantify existing procedures and to evaluate the validity of new technology. One of our hospitals (Southern Tohoku Research Institute for Neuroscience) has annually produced approximately 100, 000 sheets of film radiographs, resulting in a great deal of serious problems, particularly lack of space for storage. In addition, the overall averaged ratio of selected key films to total files of radiographs was estimated to be 16.3% for hospitalized patients during the past 5 years. It is assumed that, by means of digital storage on this selective basis, radiographic storage problems can be solved with the use of the current data base system which efficiently handles image information involving patient care. In the practical view another substantial improvement in this work is the high functionality of the system, with which radiographic images coupled with a variety of clinical data on patients can be stored in a descriptive fashion.
AIHARA, K. and INUI, J. Nitrendipine Facilitates Recovery of Cerebral BloodFlow, EEG and Metabolites Following Cerebral Ischemia in Anesthetized Rabbits. Tohoku J. Exp. Med., 1991, 165 (1), 13-24-The effect of nitrendipine, an antihypertensive calcium antagonist, on the impairment of cerebral blood flow and EEG observed after 10-min complete cerebral ischemia in anesthetized rabbits was compared with those of nicardipine. The ischemia was produced by neck tourniquet in combination with hypotension (50-60mmHg). Blood flow was measured by hydrogen-clearance method. Transient reactive hyperemia was observed immediately after the cessation of ischemic procedure, and was followed by a decrease in blood flow in the range of 58-73% of corresponding basal values in the total brain, cortex and thalamus. The postischemic decrease in blood flow was suppressed when nitrendipine (0.3-1mg/kg) or nicardipine (3-10mg/kg) was given intraduodenally before ischemia. The postischemic decrease in total intensity and frequency index in EEG recovered rapidly when nitrendipine was pretreated, whereas the recovery of EEG parameters was not obtained by the nicardipine pretreatment. These results suggest that the effect of nitrendipine and nicardipine on the postischemic cerebral blood flow may be due to the inhibition of calcium-induced contraction in cerebral vessels, whereas the discrepancy between the effects of these agents on EEG may not be due solely to the improvement in cerebral circulation. Furthermore, the improvement in postischemic cerebral energy metabolism was confirmed by nitrendipine pretreatment (0.3mg/kg).
TOKI, T. and YAJIMA, A. Immunohistochemical Localization of CarcinoembryonicAntigen (CEA) in Squamous Cell Carcinoma of the Uterine Cervix:Prognostic Significance of Localization Pattern of CEA. Tohoku J. Exp. Med., 1991, 165 (1), 25-32-Immunohistochemical localization of carcinoembryonic antigen (CEA) was investigated with 100 cases of invasive squamous cell carcinoma of the uterine cervix in order to evaluate the relationship of the positive pattern of CEA to the prognoses of patients with this neoplasm. Positive staining for CEA using peroxidase-antiperoxidase method was noticed in all tumors studied. The localization patterns of CEA were divided into the following four types depending on the location of CEA-positive areas in cancer nests: central type, surrounding type, diffuse type, and focal type. Survival curves based on calculation by the method of Kaplan-Meier showed that prognosis of patient was most excellent in the disease of central type, which was followed by surrounding type, diffuse type, and focal type, in this order. The survival curve for central type was significantly better than those for diffuse type and focal type (p<0.05) as assessed by Log rank test. In squamous cell carcinoma of the cervix, consequently, prognosis of patient might be favorable when CEA is demonstrated in the center part of cancer nests by an immunohistochemical technique.
KUNORI, T., TSUCHIYA, T., ITOH, J., WATABE, S., ARAI, M., SATOMI, T., TAKAKURA, K. and YAMAGUCHI, H. Improvement of Postoperative Hypocalcemiaby Repeated Allotransplantation of Parathyroid Tissue without Anti-RejectionTherapy. Tohoku J. Exp. Med., 1991, 165 (1), 33-40-Long lastisng postoperative hypocalcemia, an uncomfortable complication of a thyroid operation for hyperthyroidism, was treated with allotransplantation of parathyroid tissue. Small pieces of the parathyroid tissue offered from two unrelated donors were transplanted to an 18-year-old male with severe postoperative hypoparathyroidism. Prednisolone was given for 2 days, but no other immunosuppressive drugs were used. The remaining tissue was stored in frozen for the repeat transplantation. The functional activity of the frozen tissue was determined by the production of parathyroid hormone in the tissue culture medium adjusted to appropriate concentration of calcium. Loss of the graft function, probably due to rejection, was supplemented with repeated grafting. Hypocalcemia was improved by three times of transplantation using frozen tissue (once) and fresh tissue (twice). This preliminary trial demonstrates that the tissue transplantation of the parathyroid gland is effective to lessen the symptoms and medication of postoperative hypoparathyroidism.
KIKUCHI, Y., KOIZUMI, M., SHIMOSEGAWA, T., KASHIMURA, J., SUZUKI, S. and TOYOTA, T. Islet Amyloid Polypeptide has No Effect on Amylase Release fromRat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP. Tohoku J. Exp. Med., 1991, 165 (1), 41-48-Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11M), secretin (0.5×10-6M) or carbachol (10-6M), or by a maximal dose of vasoactive intestinal peptide (10-8M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.
TAKAHASHI, Y., ISHII, N., LUE, T.F. and TANAGHO, E.A. PharmacologicalEffects of Adenosine on Canine Penile Erection. Tohoku J. Exp. Med., 1991, 165 (1), 49-58-We investigated the pharmacological effects of adenosine on canine penile erection in vivo. Intracavernous injection of adenosine induced a full erection, which was unaffected by hexamethonium and atropine. The erectile response induced by adenosine was augmented by intracavernous pretreatment with dipyridamole and markedly depressed by theophylline. The erectile response induced by cavernous nerve stimulation was also enhanced by dipyridamole and reduced by theophylline. The combination of acetylcholine and adenosine, when injected intracavernously, induced a full erection, and the effect of these drugs was not additive but synergistic. These results suggest that penile erection induced by adenosine is due to a direct relaxing effect on cavernous smooth muscle. We propose that adenosine plays a cooperative role with acetylcholine and also has a physiological role as a neuromodulator in canine penile erection.
INOMATA, N., YOSHIDA, H., AOKI, Y., TSUNODA, M. and YAMAMOTO, M. Effects of Ethyl 4-chloro-2-methylphenoxyacetate on Hepatic Peroxisomal Enzymesin Rats. Tohoku J. Exp. Med., 1991, 165 (1), 59-61-Effects of ethyl 4-chloro-2-methylphenoxyacetate (MCPA) and clofibrate on the activities of hepatic lipid metabolizing enzymes in the peroxisomal fraction from rat liver were studied, and these drugs were found to potentiate the peroxisomal enzyme activity. The results imply that MCPA affects lipid metabolism as well as clofibrate.
CHIBA, S. and TSUKADA, M. Potentiation of Acetaldehyde-Induced Vasoconstrictionsby Cocaine in Isolated and Perfused Arteries. Tohoku J. Exp. Med., 1991, 165 (1), 63-65-Vasoconstrictor responses to acetaldehyde (AA), norepinephrine (NE) and tyramine were investigated in isolated, perfused canine intermediate auricular (ear) arteries. Single injections of small doses of AA (1-3 μmol) induced a vasoconstriction in a dose-related manner and showed no tachyphylaxis. Cocaine significantly inhibited tyramine-induced vasoconstrictions, but NE- and AA-induced vasoconstrictions were rather significantly enhanced.