Abstract
NAGASHIMA, Y., MIYAGI, Y., SUMINO, K., OHAKI, Y., UMEDA, M., OSHIMURA, M. and MISUGI, K. Characterization of Experimental Rat Nephroblastoma and Its Cell Line. Tohoku J. Exp. Med., 1992, 168 (2), 303-305- Rat nephroblastoma (Wilms' tumor) was induced by transplacental administration of N-ethyl- nitrosourea (ENU). The induced renal tumors were histologically compatible with human nephroblastoma. A cultured cell line (ENU-T-1) established from a xenotransplant, showed similar morphological and biological features to cultured embryonal kidney cells. Introduction of normal human chromosome #11 (#11) bearing Wilms' tumor suppressor gene(s) (WT) suppressed colony-forming ability on soft agar plates (CFA) but tumorigenicity of EMU-T-1 was not affected. Whereas tumorigenicity of human nephroblastoma cell line, SK-NEP-1 was completely suppressed, CFA was unchanged. These facts indicated that pathogenenetic mechanism is different between human and experimental rat nephroblastomas.- nephroblastoma (Wilms' tumor); transplacental carcinogenesis; cell line; chromosome transfer
