A Macrophage Differentiating Factor Derived from Human T Cell Line HUT102 Acting on a Mouse Myeloid Cell Line M1

Abstract

KANNO, H., NOSE, M., NIKI, T., MIYAZAWA, M. and KYOGOKU, M. A Macrophage Differentiating Factor Derived from Human T Cell Line HUT102 Acting on a Mouse Myeloid Cell Line M1. Tohoku J. Exp. Med., 1993, 171 (1), 43-52-Human T cell leukemia virus type I-transformed T cell line HUT102 constitutively secreted soluble factors which induced differentiation of a murine myeloid leukemic cell line, M1, to increase the immune complex-binding and/or phagocytizing capacity. This macrophage differentiating factor(s) (MDF) was purified from the culture supernatants of HUT102 cells by using several steps of column chromatography and novel immune-adherence and/or immune-phagocytic assays. The finally purified MDF activity was detected in the fraction that consisted of 40, 000- and 45, 000-molecular weight molecules. Antibodies specific for human interleukin-6 or for human granulocyte-colony stimulating factor, both of which have differentiation-inducing activity on M1 cells when used as a single factor, could not neutralize the MDF activity. These findings suggest that the 40, 000- and/or 45, 000- molecular weight molecules in the HUT102 cell products may be possible novel differentiation-inducing factors acting on a murine macrophage lineage across the species barrier.