Abstract
ISHIDOYA, S., FUKUZAKI, A. and ORIKASA, S. Localization of Several G-Protein Subunits in Human Kidney. Tohoku J. Exp. Med., 1994, 172 (3), 263-273-Localization of guanine nucleotide binding protein (G-protein) α chains in human kidney was examined using immunoblotting and immunocytochemical techniques. Immunoblot studies revealed Gαs, Gαi1, 2 and Gαi3 proteins in membrane fractions of glomeruli, cortical tubules and medullary tubules. Immunocytochemical studies confirmed the presence of these G-proteins in several parts of human nephron. The results demonstrate the presence of a large amount of G-protein α subunits in human nephron segments. We speculate that G-protein in human kidney may play an important physiological role well-defined in other mammmalia.
