Abstract
TAKAGI, S., NAITO, E., YAMANOUCHI, H., OHTSUKA, H., KOMINAMI, R. and YAMAMOTO, M. Mutation of the p53 Gene in Gallbladder Cancer. Tohoku J. Exp. Med., 1994, 172 (3), 283-289-Genetic alteration of the p53 gene was examined in tissue specimens of primary gallbladder cancer (GBC) using PCR-single strand conformation polymorphism (SSCP) and direct sequencing analyses. The p53 gene mutation was detected in five of the 16 GBC cases examined (31%). All five cases showed single point mutations; four were mutations resulting in amino acid substitution and the other was one base-pair deletion. Of the five mutations detected, four occurred at evolutionarily conserved regions and the remaining one at a well conserved region among species. Our result is similar to those of previous reports for various human cancers in mutation scattering over four of the five conserved domains.
