Abstract
KAMBE, M., Rou, K. and TACHIBANA, T. Differences in Immune Responses to Tumor Induced in Syngeneic Hosts by Injection of Hybrid and Parental Tumor Cells. Tohoku J. Exp. Med., 1994, 174 (1), 71-83 - Immunization of C3H/He mice with L-FM3A#2 hybrid cells, made by fusion of ascitic mammary carcinoma FM3A#2 cells with 8-azaguanine resistant LAG cells, both of C3H/He mouse origin, resulted in spleen T cell-dependent resistance to the parental FM3A/R cells. These spleen T cells, purified by passing through a nylon fiber column, could be demonstrated to have Thy-1.2 and Lyt-2.1 antigens, and not L3/T4 antigens. After immunizing with irradiated FM3A/R cells, cytotoxic cells other than cytotoxic T lymphocytes (CTL) appeared, these presumably being nonphagocytic macrophages or polymorphonuclear cells. In this case, anti MM antiserum was generated at an earlier stage than when mice were immunized with the L-FM3A#2 cells. The cytotoxic mechanism is discussed as to the significance of the surface antigen.
