Volume 177 (1995) Issue 1 Pages 61-71
SAIJYO, S., KUDO, T., SUZUKI, M., KATAYOSE, Y., SHINODA, M., MUTO, T., FUKUHARA, K., SUZUKI, T. and MATSUNO, S. Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1. Tohoku J. Exp. Med., 1995, 177 (1), 61-71 - A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologic allydiagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.