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Volume 177 (1995)
- Issue 4 Pages 271-
- Issue 3 Pages 183-
- Issue 2 Pages 93-
- Issue 1 Pages 1-
Volume 177, Issue 1
Displaying 1-8 of 8 articles from this issue
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TAKAHISA OTA, MASAHIKO MUTO, JUNJI NAKANO, SUMIKO HAMANAKA, REIKO IRIE ...1995 Volume 177 Issue 1 Pages 1-12
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOTA, T., MUTO, M., NAKANO, J., HAMANAKA, S., IRIE, R. and ASAGAMI, C. Immunohistological Reaction Mechanism of Anti-Monosialoganglioside Monoclonal Antibody, MAb 202, Showing Predominant Cytotoxicity for Malignant Melanoma. Tohoku J. Exp. Med., 1995, 177 (1), 1-12-Mouse monoclonal IgM antibody (MAb 202) can cause melanoma cell necrosis in vivo. We analysed its immune mechanism in three melanoma patients to whom MAb 202 was administered. After the MAb 202 administration, histopathological analysis showed necrosis of melanoma cells expressing only GM3 in two patients. Another patient carrying both GM3 and GD3 showed infiltration of lymphocytes within the tumor nest but no tumor cells or nest necrosis. Immunohistological examination using anti-mouse IgM antibody revealed MAb 202 bound on the surface of melanoma cells in two patients but not in the third (positive for both GM3 and GD3). In vitro, MAb 202 reacted with the melanoma cells of the same two patients, but not with any other tissues of these individuals. We found no reaction of MAb 202 to non-melanoma cells including normal melanocytes and glia cells. Our trials suggest, 1) MAb 202 reacts directly to monosialogangliosides on the melanoma cell surface and then leads to the cytotoxicity reaction, or 2) MAb 202 induces lymphocyte infiltration and possibly then promotes the secretion of some cytokines.View full abstractDownload PDF (6728K) -
SATOSHI MITARAI, KAZUNORI OISHI, MASASHI FUKASAWA, HIROSHI YAMASHITA, ...1995 Volume 177 Issue 1 Pages 13-23
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMITARAI, S., OISHI, K., FUKASAWA, M., YAMASHITA, H., NAGATAKE, T. and MATSUMOTO, K. Clinical Evaluation of Polymerase Chain Reaction DNA Amplification Method for the Diagnosis of Pulmonary Tuberculosis in Patients with Negative Acid-fast Bacilli Smear. Tohoku J. Exp. Med., 1995, 177 (1), 13-23-We evaluated the sensitivity and specificity of polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis among 109 patients who were suspected to have active pulmonary tuberculosis (PTB) and showed negative acid-fast bacilli (AFB) smears in a total of 393 samples of sputum (169), gastric aspirate (134), and urine (90) which fulfilled different criteria for the positivity of PCR. The patients were subsequently divided into one group of active PTB composed of 15 patients with definite PTB and 43 patients with highly susp ectedPTB, and another group of 51 non-active PTB patients. The PCR assay using samples of sputum and gastric aspirate proved to be specific for active PTB. The PCR method for diagnosis of active PTB using sputum samples was sensitive (97.8%) but lacked specificity (27.0%) when regarded as PCR positive when at least one positive reaction was obtained among all samples examined. However, the PCR of gastric aspirate demonstrated a sensitivity of 63.4% and a specificity of 76.7%. Our data supports that the PCR method for detecting active PTB in AFB smear negative patients using gastric aspirate shows markedly improved sensitivity over the conventional method (25.9%), although it still lacks specificity. PCR assay for M. tuberculosis using multiple samples of gastric aspirate in conjunction with careful clinical observations for the presence of active infection is essential for the diagnosis of active PTB among patients with negative AFB smear.View full abstractDownload PDF (1239K) -
MASANORI YAMAMOTO, HATSUKI HIBI, KOJI MIYAKE1995 Volume 177 Issue 1 Pages 25-37
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAMOTO, M., HIBI, H. and MIYAKE, K. Effects of Alpha-Blocker on Daily Testicular Sperm Production and Sperm Concentration, Motility, Intraluminal Pressure and Fluid Movement in the Rat Epididymis. Tohoku J. Exp. Med., 1995, 177 (1), 25-37-This study was undertaken to investigate whether alpha-blocker has an effect on daily testicular sperm production and epididymal sperm parameters, intraluminal pressure and fluid movement in the rat cauda epididymal tubules. Sprague-Dawley rats were given daily oral doses 1mg/kg, 2mg/kg, or 10mg/kg of Bunazosin, which was a selective alpha-Blocker, for three months. Intraluminal fluids were collected from the cauda epididymidis using a micropuncture technique. Sperm concentration was determined with a sperm concentration microassay. Motility estimates were made by measurements of linear distance traveled by sperm in the diluent. Epididymal intraluminal pressure was measured through a micropunctured pipette connected to a micropressure transducer. Intraluminal fluid movement was examined by measurement of the distance whic hSudan Black stained mineral oil moved along the epididymal tubules. Outside diameters of seminiferous and epididymal tubules were measured by light microscopy. Epididymal sperm concentrations were significantly increased after administration of alpha-Blocker. Progressive motility of spermatozoa was not significantly altered by alpha-blocker. Intraluminal pressures were significantly reduced and intraluminal fluid movement rates were significantly increased by alpha-blocker. Daily sperm production was significantly increased by alha-blocker. Outside diameters of seminiferous and epididymal tubules were significantly distended when treated with alpha-blocker. These results demonstrate that alpha-blocker has an effect on spermatogenesis and sperm transport in the rat testis and epididymis.View full abstractDownload PDF (1531K) -
TAKANORI SUZUKI, KOHEI KUROKAWA, KAZUHIRO SUZUKI, KAZUHISA MATSUMOTO, ...1995 Volume 177 Issue 1 Pages 39-48
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, T., KUROKAWA, K., SUZUKI, Kz., MATSUMOTO, K. and YAMANAKA, H. Histological and Immunohistochemical Changes after Transurethral Balloon Laser Hyperthermia in the Canine Prostate. Tohoku J. Exp. Med., 1995, 177 (1), 39-48-Transurethral balloon laser hyperthermia (TUBAL-H) for the prostate was performed in a canine model. Eleven normal and hyperplastic prostates were heated at between 40 to 45°C for 30min. Compared to the pretreatment weight, the prostatic weight significantly increased immediately after treatment and significantly decreased at 4 weeks later, but was not significantly different after 8 weeks. Histologically, shedding of epithelial cells was observed immediately after treatment, although, coagulonecrotic tissue was not seen. After 8 weeks, atrophic changes of epithelial cells were observed at the inner portion of the prostate. By immunohistochemical analysis, epithelial cells expressing apoptosis related antigen (Ley) were observed at the inner portion of the prostate from immediately after treatment until 4 weeks later. The atrophic epithelia and the expression of apoptosis in the prostate gland were pathological changes induced by TUBAL-H. From the present data, it is suggested that TUBAL-H combined with radiation or administration of anti-cancer drugs may be effective for prostate cancer.View full abstractDownload PDF (2982K) -
KAZUTOSHI SATO, TOKIHISA KIMURA, KOZO OTA, MASARU SHOJI, MASAHIRO OHTA ...1995 Volume 177 Issue 1 Pages 49-60
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, K., KIMURA, T., OTA, K., SHOJI, M., OHTA, M., YAMAMOTO, T., FUNYU, T. and ABE, K. Changes in Plasma Vasopressin Levels and Cardiovascular Function due to Postural Changes in Diabetic Neuropathy. Tohoku J. Exp. Med., 1995, 177 (1), 49-60-Decreases in blood pressure are well known to increase the release of vasopressin. Studies were carried out to investigate whether vasopressin responses to postural changes in blood pressure are maintained in diabetic patients with orthostatic hypotension [DM-OH(+)] as well as non-diabetic patients with orthostatic hypotension [nonDM-OH(+)] and these responses were compared with them observed in normal subjects and diabetic patients without orthostatic hypotension [DM-OH(-)] . After 30min in the supine position, the upright posture for 40min was maintained and then the supine for 10min. Blood pressure and heart rate (HR) were measured every 5min and plasma vasopressin levels (plasma AVP) were determined every 10min. In normal subjects and DM-OH(-), mean arterial blood pressure (MABP) did not change, but HR increased significantly by the upright position. Plasma AVP did not change in these group. On the other hand, in DM-OH(+) MABP fell abruptly and remained to decrease during the upright posture. The HR responses in this group, however, were similar to those in normal control and DM-OH(-). Plasma AVP in DM-OH(+) significantly increased only at 30min during upright. These increases were significantly greater than those in normal and DM-OH(-). There were significant correlation in changes in MABP (ΔMAP) and plasma AVP (ΔAVP) in DM-OH(+) (ΔAVP=-0.13 MABP+1.5, r=-0.32, p<0.01). Relationship between ΔMABP and ΔAVP in nonDM-OH(+) was similar to that in DM-OH(+). It is concluded that AVP responses to orthostatic hypotension in diabetic and nondiabetic neuropathies were attenuated, but heart rate responses in these patients ware well reserved.View full abstractDownload PDF (1252K) -
SUSUMU SAIJYO, TOSHIO KUDO, MASANORI SUZUKI, YU KATAYOSE, MASAO SHINOD ...1995 Volume 177 Issue 1 Pages 61-71
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAIJYO, S., KUDO, T., SUZUKI, M., KATAYOSE, Y., SHINODA, M., MUTO, T., FUKUHARA, K., SUZUKI, T. and MATSUNO, S. Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1. Tohoku J. Exp. Med., 1995, 177 (1), 61-71 - A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologic allydiagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.View full abstractDownload PDF (3366K) -
TAKEHARU FUNYU, TOKIHISA KIMURA, TADASU YAMAMOTO, KOZO OTA, MASARU SHO ...1995 Volume 177 Issue 1 Pages 73-87
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUNYU, T., KIMURA, T., YAMAMOTO, T., OTA, K., SHOJI, M., OHTA, M., SATO, K. and ABE, K. Role of Atrial Natriuretic Peptide in Interleukin 1β-Induced Natriuresis in Conscious Rats. Tohoku J. Exp. Med., 1995, 177 (1), 73-87 - To assess whether atrial natriuretic peptide (ANP) plays a role in the natriuresis induced by interleukin 1β (IL-1β), the following experiments were carried out. Experiment (Ex) I: IL-1β (7.5μg/kg BW) was given intravenously (i.v.) in conscious hydrated rats (n=6). Plasma ANP, vasopressin (AVP) osmolality (Posm), Na and K, urine Na (UNaV) and K excretion (UKV), osmolality and flow (UF), and mean arterial blood pressure (MABP) and heart rate were simultaneously determined. In the control group (n=6), the drug was omitted, and the same protocols were carried out. Ex II: Three mg/kg BW of the specific ANP antagonist, HS-142-1 (HS), was administered i.v. and then, IL-1β (7.5μg/kg BW) was given i.v. (n=6). In the HS alone group (n=6), IL-1β was omitted. The experimental protocols were the same as those in Ex I. IL-1β increased significantly plasma ANP and AVP and UNaV, but not UF, accompanied by decreases in Posm and UKV and increases in MABP (ExI). HS inhibited the natriuresis mediated by IL-1β, despite increases in plasma ANP and had no influence on plasma AVP and MABP. In the control (ExI) and HS alone (EXII) groups, these parameters did not change, except for decreases in Posm in both groups and increased plasma ANP in the latter. These results suggest that plasma ANP may play an essential role in the IL-1β-mediated natriuresis.View full abstractDownload PDF (1408K) -
YOSHIAKI SAKURAI, JING-HUA LI, SHOKO KOMATSU, HIROSHI SAITO, SUESABURO ...1995 Volume 177 Issue 1 Pages 89-92
Published: 1995
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAKURAI, Y., LI, J., KOMATSU, S., SAITO, H., ABE, S., YAMADA, A., NISHIKORI, M., TSUJI, I. and HISAMICHI, S. The Secular Trend in the Incidence of Subarachnoid Hemorrhage in Miyagi, Japan: 1979-1990. Tohoku J. Exp. Med., 1995, 177 (1), 89-92 - Since 1978, a stroke registry has been carried out in Miyagi Prefecture (2.2 million in population), Japan. Approximately 2, 000 cases of stroke were annually registered through 16 hospitals which have cerebrovascular disease units. The incidence of subarachnoid hemorrhage was 17.4 per 100, 000 population in 1990. The incidence increased gradually during early 1980's and reached plateau during late 1980's. Furthere, the trend in the incidence of subarachnoid hemorrhage is compared with those in other communities in other countries as well as Japan.View full abstractDownload PDF (343K)
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