Influence of Hydrogen Ion Concentration on the Tissue Respiration of Mouse and Rabbit in Vitro, Attention being Paid to the Buffer Potency of the Tissue

Abstract

Influence of hydrogen ion concentration of the medium on the liver tissue of mouse, chiefly on the kidney tissue of rabbit was in-vestigated, attention being specially paid to the buffer potency of the tissue, that is, power of regulating the hydrogen ion concentration of the surrounding medium by the tissue cell.
Method for tissue respiration was that of Warburg (1923, 1924).
Hydrogen ion concentration of Ringer's solution was potentio-metrically measured before and after the manometric measurement.
(1) Oxygen consumption of liver tissue of mouse was greater, the greater the pH was between 5.664 and 7.438.
The pH of the Ringer's solution increased on acid side and de-creased on basic side.
(2) Oxygen consumption of kidney tissue of rabbit, both corti-cal and medullar, was greater the greater the pH was between 5.055 and 6.900, and that at 7.611 was not different from that at 6.819.
The pH of the medium (5.460-7.363) increased after 2 hours' manometric experiment, and in the case of 3 hours' experiment, the acid medium (5.055-6.819) became more basic and the basic medium more acid.
(3) Oxygen consumption and carbon dioxide output of kidney tissue in CO₂-bicarbonate Ringer's solution with acid pH (6.1) and basic pH (8.2) was much smaller than those in the bicarbonate free Ringer's solution buffered with phosphate mixture.
The Ringer's solution shifted its acid pH towards basic side and the basic pH towards acid side.