Identification by Isolation of Sugar Components of a Pusedomucin Preparation

Abstract

A mixture of pseudomucin (Maki) and two ordinary simple proteins (obtained from a viscous ovarial cyst fluid by the procedure of Hammarsten for preparing his pseudomucin) was hydrolyzed and fractioned to isolate the sugar components. And galactose and L-fucose were isolated as a-methylphenylhydrazones, and the latter identified also as p-toluenesulfonyl-hydrazone and benzylphenylhydrazone. In addition, glucosamine-and chondrosamine-hydrochloride could be separated, but no sugar else. The present experiment afforded a proof of the findings by paper chromato-graphy in the foregoing report of this series of investigations.