Abstract
1. The carcinomatous ascitic fluids with primary tumor in stomach and the non-cancerous were fractioned, and the proteins remaining in solution on electrodialysis were purified from the crude products combined separately according to the carcinomatous or non-cancerous nature of the fluids.
2. The carcinomatous and the non-cancerous protein (preparations) both proved homogeneous electrophoretically and showed no noteworthy difference in heat-coagulability, precipitability: by alkaloid precipitants, chemical qualitative reactions, analyses, ' specific refraction and acid dye combination.
3. The former protein underwent almost no alteration in reactivity (precipitin reaction) with its antiserum, absorbed with the latter protein, by tryptic digestion, treatment with formaldehyde and heating.
4. The carbohydrate moiety of the two proteins (The protein from a liver-cirrhotic ascites was used.here as the non-cancerous) were separated after alkaline cleavage of the proteins. The preparations resembled each other qualitatively (test-tube test and paper partition chromato-graphy of the sugar components), quantitatively (The one from the carcinomatous protein contained somewhat less amino acids) and electrophoretically.
5. The carbohydrate preparation from the carcinomatous protein proved to act as the determinant group specific to that protein by the precipitin reaction with an immune serum of that protein, absorbed with the non-cancerous protein.
6. Judging from kinds of sugar component of the carbohydrate preparations, it is plausibly claimed that ascitic fluids can not be mere filtrates of blood serum.
Through the Grant Committee for Scientific Researches was given a grant from the Education Department in aid to us. H. Masamune.
