Abstract
1. An electrophoretical sedimentaion apparatus was devised for preparative
Urinary Group A, B and O Substances-II
fractionation.
2. Blood group glucidamins A, B and O, which were essentially homogeneous electrophoretically, were isolated from human urine by the electrophoretical sedimentation method, and investigated chemically and physically.
3. The A, B and O preparations pretty resembled one another in chemical composition, but differed in glucosamine/galactosamine ratio, and L-fucose-, sialic acid-, histidine- and arginine-content.
4. The gap areas in retention analysis differed from one group sub-stance to another in accord with the various hexone bases contents.
5. The substances taken up in veronal buffer of pH 8.6 disagreed with one another with respect to the boundary mobility in electrophoresis.
6. The significance of the discrepancies in composition is discussed in reference to variance of blood group activity.
Through the Grant Committee for Scientific Researches the Ministry of Education gave a grant in aid to us, which is gratefully acknowledged. H. Masamune.
