Studies on Experimental Influenza in Mice
IV. Growth Characteristics of the PR8 Virus in mouse Tissues Explanted in Vitro
1960 年 72 巻 4 号 p. 338-347
詳細
1960 年 72 巻 4 号 p. 338-347
The PRS strain of influenza virus was examined on the growth ability in three types of tissue cultures derived from various mice organs.
In Maitland type culture, the release of virus into a medium was not detectable with tissues of brain, lung, liver, kidney, spleen, pancreas and muscle. Whereas the excellent proliferation was proved with chicken chorioallantoic membrane examined under the same condition. When these tissues were placed on Zinsser's agar slant, viral multiplication was found only with lung tissues. However, the amount of virus here found was almost one tenth of that obtained with chick embryo and chicken chorioallantoic membrane used as the control in the same way.
Roller tube cultures were carried out with explants derived from various mouse organs obtained from 3-day-old mice. Reasonable growth of the cell eligible for viral growth study was obtained with lung, liver, kidney, spleen, pancreas, heart and muscle. When they are infected in vitro, growth of virus was found in tissues of lung, liver and kidney in concordant with the result obtained in animal experiment. However, in tissues of spleen any growth signs were not obtained. Daily change of the medium was favourable in detecting the release of virus from the hug tissue into a medium.
