Hydrazinolysis of Monosaccharides
1962 年 77 巻 3 号 p. 213-222
詳細
1962 年 77 巻 3 号 p. 213-222
1. Hydrazinolysis of monosaccharides resulted in the degradation of 47.5 per cent of N-acetylglucosamine, 32.7 per cent of N-acetylgalactosamine, 20.9 per cent of galactose, 12.9 per cent of rhamnose and 43.5 per cent of glucuronic acid, respectively.
2. Small amounts of 2, 5-anhydrosugars, besides other unknown compounds, were found in all the reaction products of the hydrazinolysis of the monosaccharides and small amounts of pentoses and hexoses were also detected in some of the reaction products.
3. All the hydrazinolyzed products of the monosaccharides, more or less, contained the nitrogen, which was unable to determine by the Van Slyke procedure.
4. Almost all of the hexosamines and some of the other sugars in the hydrazinolyzates of the monosaccharides were absorved on Amberlite IIt 120 (H+) resins and were easily recovered by the elution with hydrochloric acid or sulfuric acid.
5. Per cent contents of the hexosamines, which could be determined as 2, 5-anhydrohexoses by the direct deamination with nitrous acid, in total hexosamines in the hydrazinolyzates of N-acetylglucosamine and N-acetylgalactosamine amounted to 80.0 per cent and 71.4 per cent, respectively.
