Abstract
Three volunteers, aged 20, 25 and 27 were experimentally infected with an amniotic culture fluid of fresh influenza B isolate.
Two cases, which previously had low HAT antibody against the used virus, did not develop influenza B antibody, suggesting failure of the virus infection. All specimens examined throughout the course of infection by an immunofluorescent technique did not show accumulation of virus antigens.
Only one case, which previously had no detectable antibody against influenza, B, developed typical signs and symptoms of influenza. At 48 hours after infection, fluorescent cells were detected by the immunofluorescent technique in the preparations from nasal scrubs. Fluorescent cells were successively found in the nasal scrub specimens up to 120 hours after the infection. Whereas, no fluorescent cells were found in the specimens obtained from nasal washings, throat scrubs, and throat washings throughout the course of infection. First discovery of fluorescent cells was 8 hours ahead the temperature rise and 48 hours ahead the first virus isolation.
