Abstract
Autofluorescence of pathogenic fungi was investigated. Smears were made from the surface of the growth on 4% glucose agar and were fixed by heating, absolute alcohol or formalin, and were placed under the fluorescence microscope.
The fungi tested showed various kinds of autofluorescence. There were considerable differences in brightness or in color tone of autofluorescence due to the difference of species, but none due to the difference of strains. Of all fungal species tested, Microsporum japonicum and Epidermophyton floccosum gave the most brilliant autofluorescence and its color tone was pale blue.
Fixation methods fairly affected the brightness of autofluorescence, and heating was most effective in causing the most brilliant autofluorescence.
