Mechanical activities of the estrogen dominated rabbit uterus were studied in Na- and/or Cl-deficient media in vitro. In chloride-deficient media (Cl in solution was replaced by equimolar amount of anions), marked potentiation of tonic contraction was observed. The order of the potentiating effect is; Br<NO3<I<SCN. In Na2SO4-Krebs solution, cessation of phasic contraction without any detectable potentiation in tonicity was produced. Choline-, TEA-and Li-Krebs solutions produced a marked increase in tonicity. Sucrose-Krebs solution produced a slight and progressive increase in tonicity.
Sodium and potassium movements in the isolated preparation of guinea-pig's taenia coli soaked in high KCl Krebs solution and LiCl Krebs solution were estimated. In high KCl Krebs solution, both inward and outward movements of 42K were increased in proportion to the degree of depolarization. In LiCl solution 42K uptake was reduced though 24Na uptake was not influenced, 42K efflux increased and 24Na efflux was reduced slightly. The electrical stimuli of both 1V/cm and 10V/cm were effective to increase the efflux of 42K of taenia coli immersed in normal Krebs and in K2SO4 Krebs solutions, although in the latter medium, 1V/cm stimulus was ineffective to cause contraction. 24Na efflux was also increased by 10V/cm electrical stimulation in normal and in K2SO4 Krebs solution, though the change was not so significant as 42K results.
Calcium bilirubinate, a water-insoluble salt of bilirubin forming the major component of the pigmented calcium stone, was synthesized in vitro either by addition of calcium hydroxide into solutions of sodium bilirubinate or potassium bilirubinate, or by suspending free bilirubin immediately into a solution of calcium hydroxide. In this case, it was revealed that no structural change took place in the bilirubin moiety since free bilirubin, associated with the same absorption characteristics as the standard preparation, could be recovered from the synthesized calcium bilirubinate by treatments with hydrochloric acid followed by a chloroform extraction.
Electrical stimulation of the temporal lobe was performed in chronic schizophrenic patients to treat the auditory hallucinations. During electrical stimulation, there appeared different kinds of hallucinations. The characteristics of the hallucinations produced by the temporal lobe stimulation greatly differ from those of the schizophrenic hallucinations. Visual and auditory hallucinations by the temporal lobe stimulation might depend on different pathopysiological mechanisms. The kinesthetic and vestibular hallucinations could be considered as a basis of the pathophysiological mechanism of the auditory hallucinations. The temporal lobe stimulation for the auditory hallucinations in schizophrenic patients showed some therapeutic effectiveness.
In order to clarify the genesis of hemorrhagic lipemia, the following procedures were performed in rabbits; bleedings and injections of saline, plasma, corpuscle suspension, and macromolecular substance solution, respectively, simultaneous with bleedings. Then changes in plasma lipids and blood volume were studied once a day for three days. Separate injections of identical amounts of plasma and of corpuscle suspension, respectively, corresponding to twice the amount of blood withdrawn, inhibited the development of hemorrhagic lipemia and increased the blood volume. However, injections of isotonic saline corresponding to twice the amount of blood withdrawn failed to inhibit the occurrence of hemorrhagic lipemia and also failed to increase the blood volume. Injections of macromolecular substance solution in the same amount as blood withdrawn inhibited hemorrhagic lipemia. It is concluded, therefore, that the development of hemorrhagic lipemia in rabbits cannot be ascribed solely to bleeding, plasma removal or corpuscle removal, but is due to reduction in blood volume.
Cystometric examinations were done in 20 dogs with alloxan diabetes of 7-300 days duration. In 5 cases abnormal findings were noted on the cystometrogram. Of these 5 cases, one case showed a moderate increase of the intravesical pressure and autonomous cystometrogram, while other 4 cases revealed a hypotonic bladder. In 20 cases the nerves innervating the bladder were examined histologically. In 6 cases of them the degeneration of the myelinated fibers was revealed. The degeneration occurred most commonly in the small- and medium-sized myelinated fibers. The presence of degenerated myelinated fibers was proved in the pelvic nerve in 5 cases which showed an abnormal findings on the cystometrogram. Hence, it is assumed from these findings that the vesical dysfunction due to diabetes mellitus is originated from the lesions of the nerves controlling the bladder.
Autofluorescence of pathogenic fungi was investigated. Smears were made from the surface of the growth on 4% glucose agar and were fixed by heating, absolute alcohol or formalin, and were placed under the fluorescence microscope. The fungi tested showed various kinds of autofluorescence. There were considerable differences in brightness or in color tone of autofluorescence due to the difference of species, but none due to the difference of strains. Of all fungal species tested, Microsporum japonicum and Epidermophyton floccosum gave the most brilliant autofluorescence and its color tone was pale blue. Fixation methods fairly affected the brightness of autofluorescence, and heating was most effective in causing the most brilliant autofluorescence.
Idiopathic hyperglycinemia which was discovered by Childs et al. is thought to be an inherited metabolic disorder. There is, however, no evidence that the disorder is genetically determined. Our investigation was made on glycine metabolism in the parents of a patient with this particular disorder. The fasting level of glycine and ratio of glycine to serine in serum were found to be significantly higher in the parents than in controls. Glycine loading test (0.5g/kg) indicated that the parents had to some extent a handicap of glycine utilization as compared with control individuals. These results suggest that the parents are probably heterozygous for the disorder.
Glycogensis and glycolysis in the liver from a galactosemic patient were investigated. The activity of glycogenesis in galactosemic liver showed a signi-ficantly low level as compared with that in control livers, while there was no remarkable difference in the activity of glycolysis between the patient's and control livers. It was suggested that the decrease in glycogensis in galactosemic liver was due to inhibitory effect of galactose-l-phosphate on phosphogluco-mutase process.
This is a report on three cases with massive bleeding into the digestive tract. In all cases, blood was found coming from the biliary tract via the biliary canaliculi. The hemorrhage was due to inflammatory bleeding in the Glisson's capsule caused by acute interstitial hepatitis. All patients were complicated with gallstone-like symptoms, urinary abnormal findings due to acute nephritis and impairment of the liver functions besides massive melena. They were all cured by administration of tetracycline. The author has found out that acute interstitial hepatitis could be one of the causes of massive melena.