Abstract
A cell line was obtained from the liver of neonatal dd albino mice. At the second transfer after the primary culture, cell growth of all the culture ceased. Mitotic figures were not observed in these stages, during such period medium exchange was continued every 2-3 days. The cells which attached for a while to the glass surface, became larger containing abundant granules and were gradually degenerated. About a month after the primary culture, a colony of pavement-like arrangement was observed microscopically. These cells were able to be subcultured into all subsequent generations and ultimately to produce large mass of cells. This culture has had triploid range of chromosome mode.
