The Replacement of the Active Compounds in a Serum-free Medium and the Serial Transfer of L Line Cells in it

Abstract

A serum-free medium containing albumin and lactalbumin hydrolysate as the two unidentified components was described in the previous paper. The albumin could not replaced with linoleic acid. One of the action of albumin in the serum-free medium may have an ability to inhibit tryptic digestion of surface of the cells at the time of cell dispersion treatment.
Lactalbumin hydrolysate was regularly less active than Bacto-peptone. The L line cells have been successfully adapted to the serum-free medium from the low inoculum. The problem of adaptation of cells cultured in serum-free media was discussed.