Abstract
Highly purified poliovirus possessing N reactivity (N antigen) and H antigen which was prepared by heating the former, were used to pursue the production of complement-fixing antibodies in guinea pigs. By four successive intraperitoneal injections of approximately 1.0μg of viral protein of the N antigenicity, both N and H antibodies were produced in all guinea pigs tested. By the same procedure and the same amount of viral protein of the H antigenicity, the animals produced only H antibody. However, titers of H antibody were greatly varied from animal to animal in contrast with those observed in the animals injected with N antigen. Practical purpose to obtain mono-reactive N or H antisera was accomplished by using absorption technique. and
