Urinary excretion of AICA (4-amino-5-imidazolecarboxamide) following an oral dose of AICA was greater in amount in two cases with formiminotransferase deficiency syndrome as compared with those in controls. A possibility of an impaired incorporation of exogenous AICA into purine biosynthesis in formiminotransferase deficiency syndrome is suggested.
Chemoreceptor function and reflex activity of the carotid labyrinth of the toad were electroneurographically investigated. An additional attempt was made to identify chemoreceptor cells histologically. In the carotid nerve, the emission of chemoreceptor impulses together with baroreceptor ones was confirmed. The latter were discharged only by a rise in blood pressure, while the former were discharged spontaneously and at random. In perfusion experiments, lobeline, sodium cyanide and oxygen lack were found to increase the chemosensory activity. The chemoreceptor stimulations caused an excitation of respiration, indicating that the carotid labyrinth plays an important role in the regulation of respiration in the toad. In small portions of the carotid labyrinth which were still able to send the impulses to the carotid nerve, there was confirmed the existence of a small number of special cells located in the vicinity of small blood vessels, which resembled in structure the mammalian carotid body cells.
The presence of toxic substances which inhibit erythropoiesis in uremic sera is still undetermined. We examined the effect of aceton-methylalcohol extracts of serum of nephrectomized rabbits with uremia on the erythropoiesis. The percentage red cell iron utilization was markedly reduced in nephrecto-mized rabbits with uremia. The administration of aceton-methylalcohol extracts of uremic rabbit serum resulted in a marked decrease of percentage red cell iron utilization in rats which fasted for 32 hours. However, the administration of aceton-methylalcohol extracts of normal rabbit serum did not result in any decrease of percentage red cell iron utilization in fasting rats. The administra-tion of uremic extracts caused a marked shortening of the reticulocyte life span in colehicinized rats. The percentage of erythroblasts labeled with H3-thymidine in vitro was markedly reduced in uremic nephrectomized rabbits. The administration of uremic extracts resulted in a decrease of the in vivo incorporation of H3-thymidine in erythroblasts with their increased abortion in rats which starved for 32 hours. These results demonstrate the presence of toxic substances which inhibit erythropoiesis in uremic rabbit serum.
Single unit discharges were recorded from the dissected nerves of the loath barbel in response to mechanical stimulation. The responses were divided into two types, one was phasic response and the other was a group of after-discharge. Among the fibers producing phasic response to stimuli the thin fibers had lower threshold in comparison with the thick fibers. Under the repetitive stimula-tion the barbel nerve could follow up to 70/sec at temperature of 25°C, and discharge failed to follow at 180/sec. A loath is likely to substitute the function of its barbels partly for that of its lateral-line.
The authors picked up eight particular cases from a series of so-called Banti's syndrome which they had experienced at their surgical department. Although these cases had most of clinical manifestations of this syndrome, they were con-trasted with typical cases because of their normal portal pressure. Histologically, spleens of these cases showed pulp hyperplasia of various grades but, unlike in typical cases of this syndrome, no evidence of sinus hyperplasia. Besides, changes in lymph follicles of spleens of these cases included increase in their population, appearance of a germinal center and hemorrhage in perifollicular areas. This paper describes the details of these findings and presents the authors' view that these eight cases represent an incipient phase of Banti's syndrome. Discussions are also made in this paper on the relation between Banti's syndrome in its early phase and so-called primary hypersplenism.
Highly purified poliovirus possessing N reactivity (N antigen) and H antigen which was prepared by heating the former, were used to pursue the production of complement-fixing antibodies in guinea pigs. By four successive intraperitoneal injections of approximately 1.0μg of viral protein of the N antigenicity, both N and H antibodies were produced in all guinea pigs tested. By the same procedure and the same amount of viral protein of the H antigenicity, the animals produced only H antibody. However, titers of H antibody were greatly varied from animal to animal in contrast with those observed in the animals injected with N antigen. Practical purpose to obtain mono-reactive N or H antisera was accomplished by using absorption technique. and
Effects of atropine, eserine and tetramethylammonium (TMA) on the adrenal 17-hydroxycorticosteroid (17-OHCS) secretion in dogs anesthetized with sodium pentobarbital were directly evaluated. Atropine, eserine or TMA was administered intravenously. Eserine (0.3mg/kg of body-wt.) produced a marked increase in 17-OHCS secretion and the maximal secretion rate was mostly observed at 10min after injection. TMA (1.0mg/kg) induced also a definite increase in adrenal 17-OHCS secretion, the maximal secretion rate being observed at 5 or 10min after administration. Atropine (1.0mg/kg) which had been proved to produce a marked increase in 17-OHCS secretion in conscious dogs had no effects on the secretion of 17-OHCS in dogs anesthetized with sodium pentobarbital.
After 10 months of continuous cultivation in vitro, two cell lines (HE3-2 and HE4-2 cell lines) of embryonic tissue origin from Syrian hamsters did not produce tumors upon subcutaneous implantation into the hosts. After 13 months, however, the similar implantation resulted in the development of rapidly growing transplantable fibrosarcomas. The tumor-producing capacity of HE3-2 cell line was higher than that of HE4-2 line. The chromosomal analysis of HE3-2 cell line after 10 months' culture in vitro showed hyperdiploid karyotypes (“B” karyotype) with modal value of 47, while that after 13 months showed the predominance of further altered karyotypes (“A” karyotype) with modal value of 45 to 46. On the other hand, the most population of HE4-2 cell line after 13 months in vitro contained “B” karyotypes and a minor part of it showed “A” karyotypes.
The authors previously published a theory of calculus formation that components of a calculus separated from colloidal body fluid are coagulated and solidified by electrostatic effect of electrolytes, bridging action of high- molecular-weight organic substances, and kinetic energy supplied from the organism. In support of this theory, they could produce gallstone-like concre- ments of calcium bilirubinate as well as bezoar-like coagulates of persimmon in vitro, as reported earlier. This paper is concerned with the developmental mechanism of calcium carbonate stones which occur most commonly in human being. When a suspension of precipitated calcium carbonate was supplemented with proper combination of an inorganic electrolyte and an organic polyelectro- lyte and then rotated in a flask, it eventually yielded solid concrements of calcium carbonate that resembled genuine calcium carbonate stones. Accordingly, it has been shown that the theory is valid in formation of this kind of calculi.