抄録
Analyses of the erythrocyte lipid by means of thin layer chromatography (TLC) and photodensitometry (PDM) were performed in five normal human adults, four patients with hyperthyroidism and four patients with hypothyroidism.
Lipids were extracted from washed red cells with a 2:1 (v/v) chloroform-methanol mixture. TLC of total lipids was performed on a Silica Gel G plate using a mixture of chloroform-methanol-water in a proportion of 65:24:4 (v/v). Lipid spots were identified by comparing them with standard lipid spots and quantitative analysis of each lipid was carried out by means of PDM after charring the lipid spots.
The major components of erythrocyte lipids were cholesterol (unesterified), lecithin, sphingomyelin, lysolecithin, phosphatidyl ethanolamine, phosphatidyl serine, and cerebrosides. Although there were no striking differences in erythrocyte lipids in different functional states of the thyroid, relative quantitative analyses suggested that cholesterol values were high and lecithin values low in hypothyroid-ism, and phosphatidyl ethanolamine values low in hyperthyroidism.
