Enzymatic hydrolysis of apple pulp followed by lactic acid fermentation

抄録

Apple pomace was fractionated into the water-soluble and water-insoluble materials (apple pulp). The effective saccharification method of apple pulp using PECTINASE HL from Aspergillus sp. (PECase) and CELLULASE ‘ONOZUKA’ 3S (CELase) from Trichoderma viride, was investigated in addition to a screening of favorable microorganism for lactic acid fermentation from Lactobacillus amylophilus (NBRC15881), Rhizopus oryzae (NBRC5384) and Kluyveromyces thermoteleran (AOK A0357-23). On the basis of the results, lactic acid production by R. oryzae from the mixture of water-soluble materials of apple pomace and the materials solubilized by hydrolysis of apple pulp with a mixture of PECase and CELase was carried out. Lactic acid was produced at a concentration of 572 g from 46.8 kg (wet wt.) of apple pomace at 7 day. The ratio of D- and L-lactic acids was 56:44.