Morphological Control for Aggregates of DNA Molecules Chemisorbed on the Organized Films of Ternary Comb Copolymers Containing s-Triazine Rings

抄録

We investigated adsorption behavior of DNA molecules to ternary comb copolymers containing 2-vinyl-4, 6-diamino-1, 3, 5-triazine (VDAT) at air/water interface and their molecular arrangement. A newly ternary comb copolymers as adsorption template was synthesized by radical copolymerization of VDAT, octadecyl acrylate (OA), 2-(perfluorodecyl)ethyl methacrylate (FF₁₀EMA). The IR spectrum and UV-vis spectrum of transferred LB multilayers indicates adsorption of DNA molecules by ternary copolymer templates. There were hydrogenated domains at 40–50 nm diameter scales in phase separated surface structure of Z-type monolayers. Furthermore, adsorbed DNA molecules showed segment domain at 100–200 nm scales. Fluorescence emission bands of adsorbed DNA to copolymer film showed red-shift and became sharper than the one of their so lution. It was caused by formation of aggregate of π-conjugated system in the DNA with uniform aggregation number. In the case of copolymer film containing N-vinyl carbazole (NVCz), small domain at 30-40 nm scales and DNA aggregate with various aggregation number were confirmed.