抄録
Interrelationship between glucose and lipid metabolism has been studied in rats fed high calorie diet (HCD rats) compared with rats fed control diet (CD rats), gluconeogenesis and lipogenesis of which have been previously reported by authers.
1) In vivo experiments:
The fasting blood sugar level of HCD rats was higher than that of CD rats and there was no significant difference between both K values after IVGTT. On the other hand the incorporation of glucose-6-14C into fatty acids was fortified in the carcass of HCD rats. In determining insulin like activity and NEFA, hyperinsulinism and high NEFA levels of serum and epididymal adipose tissue were seen in HCD rats.
2) In vitro experiments:
When the adipose tissue of HCD or CD rats was incubated with glucose-6-14C and insulin, it has been shown that the glucose uptake, incorporation of glucose-14C into fatty acids and NEFA release in HCD rats decreased, but the incorporation of glucose-14C into CO and glyceride glycerol and glycerol release in HCD rats did not decrease compared with that in CD rats. Also we found that epididymal adipose tissue was resistant to insulin response in HCD rats. re-esterification of NEFA.
These data suggest that glucose tolerance is not disturbed, but its hyper NEFA emia and hyperinsulinism cause the abnormal pattern of glucose metabolism in HCD rats, which accelerates obesity because of the
The difference between fatty acid synthesis from glucose in vivo and in vitro seems to be due to the total amount of adipose tissue.
