抄録
Relationship between injury of living cells and cellular membrane damages arising from freeze-thawing and freeze-drying was investigated. In yeast cells, it was demonstrated that an increase of membrane permeability was brought about by freeze-thawing and freeze-drying, at the first, and then the degradation of the membrane phospholipids followed to. By the experiments using human erythrocytes, the structural change of the membranes was supposed that it can be ascribed to the dehydration from the cells. NMR study in E. coli cell membranes demonstrated that a significant structural change of phospholipids was brought about in the logarithmic phase specimen, which is sensitive to freeze-thawing and freeze-drying. In contrast, in the stationary phase specimen, which is highly resistant to the same treatments, a very slight lipid structural change was observed. Glycerol effectively endured the lipid configuration in logarithmic phase cell membranes in the same freeze-thaw treatment.
