抄録
An Erwinia ananas ice nucleation-active gene, named inaA, was cloned which encoded a protein with 1322 amino acid residues. When Escherichia coli was transformed by introducing this gene, it acquired an icenucleating activity which was due to production of the same protein in the inclusion body form. The possibility exists that the protein, when immobilized on the surface of a film, can be used as an ice nucleator for freeze concentration of raw and/or fresh foods under a very mild condition.