p53 gene states are involved in the degree of bleomycin-induced cytotoxicity

抄録

In this study, we determined whether p53 expression affected the sensitivity of non-small cell lung cancer (NSCLC) and colon cancer cells to bleomycin. Cells were treated with various concentrations of bleomycin and cellular viability was assessed by formazan assay using CCK-8 reagents. To investigate the role of p53 in bleomycin sensitivity, we transduced cells with adenovirus expressing wild-type p53 or dominant-negative p53 and analyzed the effect of bleomycin on cellular viability. The expression of p53 and p21, as a control, was examined using Western blot analysis. Cells expressing wild-type p53 were more sensitive to bleomycin than p53-null cells in both lung cancer and colon cancer cells. In addition, bleomycin sensitivity was dramatically increased or decreased by overexpression of wild-type and dominant-negative p53, respectively. We propose that p53 plays an essential role in the response to bleomycin and may be considered a target in bleomycin therapy.