主催: 日本毒性学会
Acne vulgaris is a chronic inflammatory disorder of the sebaceous follicles. Propionibacterium acnes plays a critical role in the development of these inflammatory lesions. The present study was conducted to evaluate the antimicrobial property of honeybee (Apis mellifera L.) venom (BV) against the etiologic agents of acne vulgaris and the pharmacological activities of honeybee (Apis melifera L.) venom (BV) have been used in wound healing for centuries. Incubation of the skin bacteria P. acnes, clindamycin-resistant P. acnes, Staphylococcus epidermidis or Streptococcus pyrogenes with BV yielded the minimal inhibitory concentration (MIC). Production of inflammatory cytokines (interleukin-8 (IL-8) and tumor necrosis factor- α (TNF-α)) were examined in THP-1 cells. To study wound healing, full thickness skin defects were produced on the dorsal area of mice. The wound sizes were small in the BV group compared to the Control and Vaseline groups. The BV group demonstrated decreased TGF-β1, fibronectin and VEGF mRNA levels and increased collagen-I mRNA levels. The expressions of TGF-β1, fibronectin and VEGF proteins was significantly lower in the BV group compared to the Control group, while the expression of collagen-I was increased in the BV group as indicated by immunohistochemical staining. These data suggested that BV has effective antimicrobial and anti-inflammatory activity against P. acnes, and we suggest that BV is an alternative treatment for antibiotic therapy of acne vulgaris and significant wound healing activity.
