抄録
1-Bromopropane (1-BP) was introduced as one of new substitutes for ozone depleting compounds. 1-BP is used in multiple manufacturing processes, including vapor and immersion degreasing operations to clean metal, precision instruments, electronics, optical instruments and ceramics and used as a solvent vehicles for aerosol applied adhesives in cushion manufacture industries. Several human case studies have reported polyneuropathy and neurotoxicity in 1-BP-exposed workers. Animal studies have shown reproductive/developmental toxicity as well as central neurotoxicity. However, developmental neurotoxicity remains unclear. Here we report that 1-BP affects hippocampal excitability and sensitivity to excitotoxic compound pentylenetetrazole (PTZ) during developmental period. Pregnant Wistar rats were inhalationally exposed to 1-BP vapor from day 1 to 20 (6 h/day) with the concentrations of 0, 400 and 700 ppm. On the days of PND 13, 14 and 15, hippocampal slices were prepared from the offspring. At PND 14, stimulation/response curves of the field EPSP and the population spike (PS) were enhanced and paired-pulse ratios of PS were decreased in the hippocampal CA1 region in the 1-BP exposed groups. Systemic injection of PTZ to the PND14 offspring revealed a significant decrease the occurrence of wild running episode, tonic convulsion and status epilepticus in the 1-BP exposed groups compared to the control group. Our results suggest that prenatal exposure to 1-BP modifies the development of neuronal networks and changes excitability of the brain during the lactation period.
