抄録
We present data on peripheral blood lymphocyte subsets for a large cohort of naïve Beagle dogs of at least 6 months of age. Venous blood samples were collected from fasted animals in the morning. Immunophenotyping analysis to quantify B lymphocytes, total T lymphocytes, T CD4+ and CD8+ lymphocytes using commercial BD Pharmingen fluorescent antibody reagents (BD Biosciences, France) was performed on a Navios 10-color flow cytometer with Navios v1 software (Beckman Coulter, France). Flow count® fluorospheres were added to each sample prior to cytometry for direct determination of lymphocyte subset absolute counts. Results obtained in males and females, respectively, are presented as percentage of gated cells and mean/ul (± standard deviation) of absolute count of each lymphocyte subset: B lymphocyte percentage (21.3% & 18.5%) and absolute count (604.4±185.5 & 495.5±171.5); total T lymphocyte percentage (60.8% & 64.1%) and absolute count (1719.6±599.4 & 1728.5±334.9); T CD4+ lymphocyte percentage (41.0% & 43.6%) and absolute count (1180.4±399.7 & 1159.3±228.1); T CD8+ lymphocyte percentage (12.7% & 12.2%) and absolute count (360.2±136.6 & 324.6±106.9). Stored samples from additional animals to reach a total of 100 animals from each sex are now available and will shortly constitute the largest cohort of data on peripheral blood lymphocyte subsets in the naïve Beagle dog. There is a growing need to generate historical immunological background data of this kind to interpret the results of nonclinical immunotoxicity studies in dogs, since the dog is a major non-rodent species used in regulatory drug safety studies.
