Mechanism of acetaminophen-induced reduction in eye pigmentation in zebrafish based on transcriptome analysis

Huan WANG; Guiying KANG; Chenglong MA; Hua LIAN; Kexin ZHAO; Jiangdong XUE; Zheng NA; Jingfeng YANG; Ahmed RG; Wu DONG

doi:10.14869/toxpt.50.1.0_P1-075S

第50回日本毒性学会学術年会

セッションID: P1-075S

DOI https://doi.org/10.14869/toxpt.50.1.0_P1-075S

会議情報

主催: 日本毒性学会

会議名: 第50回日本毒性学会学術年会

開催日: 2023/06/19 - 2023/06/21

学生ポスター発表賞応募演題

Mechanism of acetaminophen-induced reduction in eye pigmentation in zebrafish based on transcriptome analysis

*Huan WANG, Guiying KANG, Chenglong MA, Hua LIAN, Kexin ZHAO, Jiangdong XUE, Zheng NA, Jingfeng YANG, Ahmed RG, Wu DONG

著者情報

*Huan WANG
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Guiying KANG
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Chenglong MA
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Hua LIAN
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Kexin ZHAO
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Jiangdong XUE
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Zheng NA
Key Laboratory of Groundwater Resources and Environment, Ministry of Education, College of Environment and Resources, Jilin University, Changchun, 130021, China.
Jingfeng YANG
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China
Ahmed RG
Division of Anatomy and Embryology, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Wu DONG
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China

会議録・要旨集フリー

詳細

抄録

Acetaminophen (N-acetyl-p-aminophenol; APAP) is one of the most widely used analgesic and antipyretic drugs. In order to investigate the toxicity of APAP, we used zebrafish embryos or larvae as model animals to test the effect of APAP on zebrafish embryos or larvae. Zebrafish embryos were exposed to 0 (control), 0.01, 0.1, 1, and 5 mM APAP from 4 hour post fertilization (hpf) to detection. Morphological changes in zebrafish were observed every 24 hpf, and behavioral changes were detected from 18 to 120 hpf. Histological changes, transcriptional analysis, and expression changes of thyroid-related genes were performed at 72 hpf. Experimental data showed that APAP caused pericardial edema in zebrafish embryos or larvae (42.7%, P＜0.05), and pigmentation decreased (66.6%, P＜0.05). Behavioral analyzes showed that APAP (0.1-5 mM) exposure also resulted in a significant increase in coiling behavior in early-developing embryos (18-32 hpf). However, when tested at 120 hpf, APAP (0.1 and 1 mM) increased the swimming distance of zebrafish larvae, while 5 mM APAP decreased the swimming distance of zebrafish larvae (100%, P＜0.05). KEGG discovery of retinol metabolism, Fanconi anemia pathway, and tyrosine metabolism. Differentially expressed lncRNAs were identified to affect pigment development (fabp4b, cyp7a1, rpe65a) as well as pigment production in the thyroid (tyrp1b, fabp4b). RT-PCR assay also showed that APAP treatment led to a decrease in zebrafish thyroid peroxidase (tpo) and thyroid hormone receptor β (thrβ) mRNA expression (P ＜ 0.05). The deleterious effects of APAP on zebrafish embryos may be due to its disruptive effect on the functional regulation of the thyroid hormone (THs) system. It is suggested should use APAP with caution.

責任著者(Corresponding author)

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