The karyotypes of seven species of the genus Semisulcospira from Lake Biwa, Japan were examined. The observed diploid chromosome number was as follows: Semisulcospira dilatata 2n=20 (14M+2SM+4T), S. rugosa, 2n=22 (16M+4SM+2ST); S. arenicola, 2n=24 (18M+4SM+2ST); S. fuscata, 2n=26 (18M+6SM+2ST); S. ourensis, 2n=28 (20M+6SM+2T), S. reticulata, 2n=28 (1M+6SM+6T), S. sp. cf. multigranosa, 2n=28 (4M+4SM+20T). The number of chromosomes in S. niponica group ranged from 2n=18 to 32, but no case of 2n=30,34 have been reported.