Abstract
The effects of X ray whole body irradiation on the riboflavin distribution in some tissues of adult male rats were observed by the paper partition chromatography. In 72 hours after a single irradiation with dose of 800r, the riboflavin concentration rose prominently in the spleen and slightly in the heart, kidneys and liver. Among three fractions of riboflavin, FAD decreased and FMN and free vitamin increased prominently in the liver, moderately in the heart and kidneys, and slightly in the spleen. The proportion of the respective fraction to the total riboflavin varied only slightly among various tissues of the irradiated rat, while the variation was considerable in the nonirradiated control. From the above results, it may be concluded that the X ray irradiation lowers the riboflavin utilization of tissues and the esterification of the vitamin in the liver and kidneys. The spleen seems, however, to take a particular attitude with regard to the effect of irradiation on riboflavin distribution.
