When an excessive amount of thiamine was loaded with perfect meals to men, the urinary excretion of the vitamin varied by the way of loading. Administration of three times a day (A) showed a larger excretion than that of once a day (B). Changing the diet to thiamine deficient one, the urinary excretion of the vitamin reached the minimum level after a few days in both conditions, but it was always higher in A than in B. This difference seems to be resulted not to difference in the leakage of the vitamin but from a lower thiamine requirement in the A condition. Loading the vitamin with meal is supposed to lower the thiamine requirement by about 20 per cent compaired with that made between meals.
