Abstract
The mechanism of the adsorption of thiamine with protein was further investigated. This reaction would occur as the one of the liquid-solid phase adsorption, and so the protein concerned in this reaction must have the solid phase, either by higher molecular weight like as globulin or by denaturation like as denaturated albumin. The treatment with p-chloromercuribenzoate showed no effect on this reaction, while that with hydroxylamine had an inhibitory action. From these results it is assumed that this adsorption would occur between the vitamin and the carbonyl groups of protein.
