抄録
The action of carbodiimide on FMN under various conditions was investigated by means of paper chromatography. (1) In case of FMN as a sole reaction component, two fluorescent spots were observed, one of which was due to the remained FMN. The another had Rf value of about 0.4 with n-butanol・pyridine・water (4 : 6 : 4) as the developing solvent. This compound (U_1), was produced by the reaction both in pyridine containing a small amount of water and in dimethylformamide, but was not produced in absolute pyridine.(2) When H_3PO_4 was added to the reaction system, three flavin compounds were formed and designated as U_2,U_3 and U_4. One of them, U_2,had Rf value similar to that of U_1 mentioned above. The other two could be produced only when an excess of H_3PO_4 or carbodiimide was used.(3) In the system which contained FMN and AMP as the reactants, four fluorescent compounds (U_5 to U_8), were detected. Rf values of U_5 and U_8 were closely resembled to those of U_2 and U_4,respectively. These results suggested that the reaction was not simple when two reaction components were contained.
