Abstract
The author has devised a histochemical method of examination of orotic acid by utilization of primary fluorescence which was remarkably intensified on addition of 10% H_2O_2,and observed the histological distribution of the orotic acid in rats. Orotic acid was observed chiefly from the intestine and excreted in bile from liver cells and in urine from kidneys. The absorbed orotic acid was found abdundantly in liver, bone marrow, testis, kidneys and brain etc. First, the orotic acid was found in the cytoplasma and then in the nucleus. Especially, in nervous cells orotic acid was mostly demonstrated in the Nissl bodies.
