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Yasuo MASE
Article type: Article
1961 Volume 22 Issue 2 Pages
129-133
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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When vitamin A acetate was irradiated by ultraviolet ray in acetone solution, a compound was obtained, and it was purified by chromatographic separation on alumina. This compound was identified as anhydrovitamin A by chromatographic behavior on alumina, by spectral properties both in ultraviolet and infrared absorptions, and by coloration with antimony trichloride in chloroform solution.
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Yasuo MASE
Article type: Article
1961 Volume 22 Issue 2 Pages
133-137
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Vitamin A alcohol dissolved in petroleum ether containing lead peroxide was oxidized into retinene in only five minutes by shaking at 26℃ and its yield was approximately 65%. Retinene was reduced to vitamin A alcohol with lithium aluminum hydride in absolute ethyl ether and its yield was approximately 40%.
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Masahide SHIROISHI, Akira HAYAKAWA
Article type: Article
1961 Volume 22 Issue 2 Pages
138-141
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Aqueous solutions of vitamin B_6 relating compounds and 4-pyridoxic acid were exposed to sunlight to compare the stability at pH 3.0,7.0 and 9.0. Pyridoxine and pyridoxamine were relatively stable in an acidic medium, while decomposition of pyridoxal occurred independently on pH value. The fact suggests that oxygen participates in the photolysis of pyridoxal. Presence of Cu ion was found to have no effect on the stability of these substances. Borate complex of pyridoxine was stable in the sunlight irradiation as well as in heating or autoclaving. Acetic acid and a reddish brown substance were separated as the decomposition products from an irradiated solution of pyridoxine. It was found that the latter substance is precipitated by the addition of acetone or phosphotungstic acid, does not contain nitrogen and reduces Fehling's solution.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
141-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Ryozi NANBA, Sosogu NAKAYAMA
Article type: Article
1961 Volume 22 Issue 2 Pages
142-146
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Intravenous injection of thiamine propyldisulfide (TPD) produces an excitatory effect on the movements of the small intestine in the dog, while thiamine preparations of the other manufacturers exert no effect. When TPD is applied to the exposed mucosa. of the small intestine, the intestinal movements above the spot applied are remarkably excited, while the other thiamine preparations has no effect on them. When 0.033 or 0.05% of TPD solution is perfused through the intestinal loop at a given pressure, the weak movements of the intestinal loop are activated and the outflow volume of fluid through the intestinal loop is enhanced. On the other hand ; when the intestinal movements are under an active condition they are accelerated, but because of increase of the muscular tone the lumen becomes narrow, with a consequent decrease in the outflow volume of fluid through the intestinal loop.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
146-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Kunihiko HATTORI
Article type: Article
1961 Volume 22 Issue 2 Pages
147-162
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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During the last few years, we have been studying on the antibody formation in vitamin B_6 deficiency. For the purpose of studying the mechanism of its diminution, in this paper, the author examined the function of the reticuloendotherial system, the cell reaction in local lymph nodes, the protein synthesis and the secondary reaction in sensitized vitamin B_6 deficient rats. The atrophy of the lymphatic tissue and inhibition of its mitosis and cell reaction to the antigen were found. Moreover, it was found no diminution in the secondary response in spite of such changes in the primary response.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
162-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Kozo YAMADA, Takayoshi FUJII, Muneaki MOTOYASU
Article type: Article
1961 Volume 22 Issue 2 Pages
163-167
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The authors examined histological changes, serum cholesterol contents, serum electrophoretic partition and lipemia clearing factor in arteriosclerotic patients and experimental atherosclerotic rabbits injected pyridoxal phosphate for long time. It was found that pyridoxal phosphate was able to prevent the arteriosclerotic changes. This seems to be due to the lypolytic factor of pyridoxal phosphate in the arterial wall with increase of serum albumin contents.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
167-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Kozo YAMADA, Kohei KAWAGUCHI
Article type: Article
1961 Volume 22 Issue 2 Pages
168-174
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The parenteral use of orotic acid in male albino rats causes fatty infiltration in the tubules, particularly in the convoluted tubules of the cortex of the kidney and peripheral parts of the lobules in the liver. The minimal dose of orotic acid producing the fatty change is 5mg/100g in a single injection and 0.2-0.04mg/100g in the repeated injections. The accumulation of fat in the kidney and the liver was also induced by the oral administration of orotic acid more than 10 mg for 15 days. An injection of orotic acid does not exert the beneficial effect on the experimental liver damage by carbon tetrachloride in rats.
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Kunio YAGI, Jun OKUDA, Yoshiko YAMAMOTO
Article type: Article
1961 Volume 22 Issue 2 Pages
175-177
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Riboflavin tetrabutyrate, riboflavin tetracaprate, and riboflavin tetrapalmitate were synthesized by our method and riboflavin tetraacetate was synthesized by the esterification of riboflavin with acetic acid anhydride. To test the possibility of the hydrolysis of these esters in living body, the authors first examined the hydrolysis of these esters by pancreatic lipase. The results showed that the above four esters tested were hydrolyzed to riboflavin and corresponding fatty acids. Among them, riboflavin tetrabutyrate was most easily hydrolyzed and followed by tetracaprate, tetraacetate and tetrapalmitate.
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Soichi NIWA
Article type: Article
1961 Volume 22 Issue 2 Pages
178-181
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Following to the studies on enzymatic hydrolysis of fatty acid esters of riboflavin by pancreatic lipase, the author examined the hydrolysis of these esters by human dijestive juice, i.e., saliva, gastric and duodenal juice. By saliva and gastric juice, these esters were not hydrolyzed. However, these esters were hydrolyzed by duodenal juice to split off riboflavin. Among riboflavin-2', 3', 4', 5'-tetrapalmitate, -tetracaprate, -tetrabutyrate and -tetraacetate, tetrabutyrate was most easily hydrolyzed, followed by tetraacetate, tetracaprate and tetrapalmitate. In the case of tetrabutyrate, the optimum pH and temperature were found at 8.5 and 40℃, respectively.
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Toshiro MATSUBARA
Article type: Article
1961 Volume 22 Issue 2 Pages
182-187
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Riboflavin content in blood and urine after the injection of riboflavin tetrapalmitate or riboflavin tetrabutyrate was determined. By intramuscular injection of riboflavin tetrapalmitate, the increase of riboflavin content in blood and in urine was not clearly observed. However, in the case of riboflavin tetrabutyrate, a slight increase of riboflavin in blood was demonstrated, and 30-40% of the injected riboflavin was excreted as free riboflavin in urine within 24 hours after the injection. These results indicate that fatty acid esters of riboflavin could be used as remedy which can be deposited in animal body.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
187-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
187-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Iwao INOUE
Article type: Article
1961 Volume 22 Issue 2 Pages
188-196
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The free amino acids contents in the liver, kidney and brain of several animal families were examined by two dimentional paper chromatogram. The test animals were bull, pig and dog, as the mammalia, snake and tortoise as the reptiles, crucian carp and tuna as the fish. It was recognized that there is the peculiar relation of free amino acids between differential families. The quantities of aspartic acid, glutamic acid, serine and glycine in the brain of cold-blooded animal are low compared with those in warmblooded animal, on the otherhand γ-aminobutyric acid is similar. The free amino acids in the viscera of reptiles are low generally. In the fishes, they are higher than those in the reptiles, and especially serine, glycine, alanine, valine+methionine and leucine+isoleucine in the fish liver are higher than thos ein the mammalia. The contents of valine+methionine and leucine+isoleucine in the tuna is comparatively high value. In the fish kidney, the contents of serine, glycine, alanine, valine+methionine and leucine+isoleucine are comparatively high also.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
196-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
196-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
196-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Chikataro KAWASAKI, Eiichi HIRAOKA
Article type: Article
1961 Volume 22 Issue 2 Pages
197-199
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The growth of L. fermenti 36 which requires thiamine for its growth was inhibited by thiamine sulfuric acid ester but not the growth of L. arabinosus and L. casei which requires no thiamine. The inhibition of thiamine sulfuric acid ester for L. fermenti was recovered competitively and specifically by thiamine, and its inhibition index was 1,000-1,500. The compound had no thiamine activity.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
199-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
199-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Eiichi HIRAOKA, Isao TOMITA, Hiroe SHIMADA
Article type: Article
1961 Volume 22 Issue 2 Pages
200-203
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The growth of L. fermenti 36 which requires thiamine for its growth was inhibited by deoxythiamine (DOB_1), deoxymethylthiamine (DOMB_1) and deoxyethylthiamine (DOEB_1), but not for the growth of L. arabinosus and L. casei which do not require thiamine. The inhibition of these compounds for L. fermenti was recovered competitively and specifically by thiamine and their inhibition index was 200-300 for DOB_1 and DOMB_1,about 500 for DOEB_1. These compounds had no thiamine activity. The order of decreasing antithiamine potency was : pyrithiamine > DOB_1≒DOMB_1 > DOEB_1 > thiamine sulfuric acid ester > oxythiamine.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
203-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
203-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Eiichi HIRAOKA, Hiroe SHIMADA
Article type: Article
1961 Volume 22 Issue 2 Pages
204-206
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Thiothiamine and thiochrome which are structurally related to thiamine, had little thiamine activity and no inhibitory effect on the growth of L. fermenti 36. Deoxy compounds and sulfuric acid esters of thiothiamine and of thiochrome had neither thiamine activity nor inhibitory effect.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
206-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
206-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
206-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
206-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Hokin KO
Article type: Article
1961 Volume 22 Issue 2 Pages
207-214
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The histopathological studies on the lesion of nervous tissues in the experimentally produced vitamin E deficient rats were performed and the results obtained were as follows. The degenerative changes were found in several nuclei of the brain and spinal cord, most marked in the pyramidal tract. Similar changes were observed in the peripheral neurons in the stomach, intestine, uterus, etc. The degeneration of nerve fibers and end-plates, which preceded the changes of skeletal muscle, were seen.
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Shotaku So
Article type: Article
1961 Volume 22 Issue 2 Pages
214-220
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The author has devised a histochemical method of examination of orotic acid by utilization of primary fluorescence which was remarkably intensified on addition of 10% H_2O_2,and observed the histological distribution of the orotic acid in rats. Orotic acid was observed chiefly from the intestine and excreted in bile from liver cells and in urine from kidneys. The absorbed orotic acid was found abdundantly in liver, bone marrow, testis, kidneys and brain etc. First, the orotic acid was found in the cytoplasma and then in the nucleus. Especially, in nervous cells orotic acid was mostly demonstrated in the Nissl bodies.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
220-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Shotaku So, Daisuke SHINTANI, Masuo YAMANISHI
Article type: Article
1961 Volume 22 Issue 2 Pages
221-222
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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The distribution of orotic acid in rats intoxicated with carbon tetrachloride was oberved with histochemical techniques. In liver, orotic acid was found in regenerating liver cells of peripheral parts of lobules. This was interpreted that the orotic acid administrated was utilized in regerneration of liver cells. From the findings it was suggested that the liver plays an important role in orotic acid metabolism.
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Yoshio TANI, Yoshiyuki YOSHIDA, Saburo FUKUI
Article type: Article
1961 Volume 22 Issue 2 Pages
223-227
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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In this paper the change in the vitamin contents was studied in the process of alcohol production by the method most widely practiced in Japan, in which submerged culture of Aspergilli was used for saccharification and Amylo process for preparation of yeast starter. Throughout the whole process, the concentrations of pantothenic acid, biotin, inositol and vitamin B_5 remained above the necessary values, though incrase or decrease due to biosynthesis or consumption by microorganisms were observed. However it must be pointed out that the amount of thiamine was very small and the ratio of thiamine to pantothenic acid was much lower than the optimal value shown in our preceding paper.
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
227-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
227-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Ryoji ASAYAMA, Yasuko MAEDA
Article type: Article
1961 Volume 22 Issue 2 Pages
228-236
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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Albino rabbits were administered either vasodilative agents (acetylcholine, benzyl imidazoline hydrochloride and Kallikrein) or vasoconstrictive agents (adrenalin and ephedrine), each alone or in combination with thiamine. The concentrations of thiamine in the blood, retina, optic nerve and choroid were estimated. In the case of an administration of vasodilative agents alone, the thiamine concentrations increased as soon as 5 minutes after the injection in some cases, reaching their peaks in 15 to 30 minutes, and began to return to normal in 60 minutes in almost all cases. A marked increase of thiamine in the optic nerve only was resulted by the administration of each vasodilative agent with thiamine. When acetylcholine was given combined with thiamine, the concentration of thiamine in the blood increased. Phosphorylation was, however, accelerated in almost all cases. The thiamine concentration in ocular tissues began to decrease in 5 minutes after adrenalin administration, reaching its minimum in 15 to 30 minutes and turned to return to normal thereafter. The administration of ephedrine resulted in a marked transition of thiamine into the ocular tissues. But after two hours, it returned to the normal value. A simultaneous application of adrenalin with thiamine resulted in an inhibition of thiamine transition from the blood to the ocular tissues. This inhibition was not observed by the application of ephedrine with thiamine. The administration of vasodilative agents results in a dilatation of the blood vessels and an increase in the amount of circulating blood in the eyeball, leading to an increased permeability in the blood retinal barrier. Furthermore it may result in an accerelated glycolysis and phosphorylation of thiamine followed by an increased production of energy in the ocular tissues. When adrenalin is given, the effect on the other endocrine glands and stress syndrome should be considered in addition to its vasoconstrictive effect. Ephedrine does not inhibit the thiamine-transfer from the blood to the retina and optic nerve, because its vasoconstrictive effect is slighter with short duration.
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
237-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
237-238
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
238-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
238-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
238-239
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
239-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
239-240
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
240-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
240-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
241-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 22 Issue 2 Pages
241-
Published: February 25, 1961
Released on J-STAGE: December 18, 2017
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