抄録
Some investigations were undertaken on improvement of culture medium for production of DBCC by cultivating Propionibacterium shermanii with added precursors. About 20% increase of cell growth was attained by omitting casamino acid and tryptose from Bernhauer's medium and by adding a kind of fish soluble (tuna extract) to the medium. It was found that glucose was more excellent carbon source than lactose or lactic acid. Using the modified medium, cultivation of Pr. shermanii in the presence of added hydroxocobalamin was made for 10 days and 1.5 g (on dry weight) of cells were obtained per 1 l of broth. More than 80% of total E. coli-activities were found to be present in the cells, mainly as DBCC form.
