Abstract
The fluorometric determination of pyridine nucleotide in human blood was investigated. After deproteinization with HClO_4,the samples were treated with alcohol dehydrogenase and isocitric dehydrogenase and then methylethylketone procedures were performed. The recoveries of both DPN^+ and TPN^+ were satisfactory. By this method the mean value of total pyridine nucleotide of normal human blood was 65.1 mμmoles/ml in ten cases and DPN^+/TPN^+ ratio was ranging from 1.1 to 1.8. In anemia patients, it was revealed that PN^+ levels were apparently decreased, however, the decrease was not recognized if the PN^+ value was corrected with hematocrit value. The DPN^+/TPN^+ ratio showed no remarkable change as compared with normal cases except one.
